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Mouse Monoclonal ICAM2 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing ICAM2 protein.

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Images

Flow Cytometry - Anti-ICAM2 antibody [B-T1] - BSA and Azide free (AB34333), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.3 - 7.5
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing ICAM2 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13598

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Alternative names

Recommended products

Mouse Monoclonal ICAM2 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing ICAM2 protein.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing ICAM2 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13598
Carrier free

Yes

Clone number

B-T1

Purity

Tissue culture supernatant

Light chain type

kappa

Concentration
Loading...
Purification notes

This antibody was purified by ion exchange chromatography. It is supplied sterile filtered through 0.22 µm and treated to remove endotoxins.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab34333 is a carrier free version of Anti-ICAM2 antibody [B-T1] ab47172.

This product was changed from ascites to tissue culture supernatant on 4th April 2 19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ICAM2 also known as IC2 or ICAM-2 is a cell surface glycoprotein with a molecular mass of approximately 55-65 kDa. It plays an important role in immune response. The protein belongs to the immunoglobulin superfamily and interacts specifically with integrin molecules. ICAM2 is expressed widely across many tissues including endothelial cells platelets and leukocytes. Its primary function involves facilitating stable adhesion between leukocytes and endothelial cells which is essential for leukocyte transmigration.

Biological function summary

ICAM2 helps in immune function by serving as an adhesion molecule. It does not form part of a larger protein complex but instead it acts independently to mediate interactions between cells. It contributes to leukocyte rolling and firm adhesion stages necessary for leukocyte extravasation. This function is important in immune surveillance and response to infection allowing immune cells to quickly migrate to sites of inflammation.

Pathways

ICAM2 operates in the leukocyte adhesion and transendothelial migration pathways. It interacts closely with integrins especially LFA-1 present on leukocytes and this interaction supports cell movement during immune responses. It also participates in signaling pathways that activate immune cells further aiding in the regulation of immune cell activity. These interactions make ICAM2 a critical component in immunological communication and signal transduction pathways.

Associated diseases and disorders

ICAM2 is associated with conditions involving immune dysfunction and inflammation. For instance abnormal expression of ICAM2 has been linked to inflammatory diseases such as atherosclerosis. Additionally it plays a role in the pathology of multiple sclerosis where it might influence the migration of immune cells across the blood-brain barrier. Its relationship with integrins highlights its potential relevance in therapeutic strategies to modulate immune responses in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Flow Cytometry - Anti-ICAM2 antibody [B-T1] - BSA and Azide free (ab34333), expandable thumbnail

    Flow Cytometry - Anti-ICAM2 antibody [B-T1] - BSA and Azide free (ab34333)

    Flow cytometry analysis staining ICAM2 in human lymphocytes using Anti-ICAM2 antibody [B-T1] ab47172 monoclonal antibody.

    This image was generated using the ascites version of the product.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ICAM2 antibody [B-T1] ab47172).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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