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AB34333

Anti-ICAM2 antibody [B-T1] - BSA and Azide free

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Mouse Monoclonal ICAM2 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing ICAM2 protein.

View Alternative Names

CD102, Intercellular adhesion molecule 2, ICAM-2, ICAM2

1 Images
Flow Cytometry - Anti-ICAM2 antibody [B-T1] - BSA and Azide free (AB34333)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ICAM2 antibody [B-T1] - BSA and Azide free (AB34333)

Flow cytometry analysis staining ICAM2 in human lymphocytes using ab47172 monoclonal antibody.

This image was generated using the ascites version of the product.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47172).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-T1

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing ICAM2 protein. The exact immunogen used to generate this antibody is proprietary information.

P13598

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

ab34333 is a carrier free version of ab47172.

This product was changed from ascites to tissue culture supernatant on 4th April 2 19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
This antibody was purified by ion exchange chromatography. It is supplied sterile filtered through 0.22 µm and treated to remove endotoxins.
Storage buffer
pH: 7.3 - 7.5 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM2 also known as IC2 or ICAM-2 is a cell surface glycoprotein with a molecular mass of approximately 55-65 kDa. It plays an important role in immune response. The protein belongs to the immunoglobulin superfamily and interacts specifically with integrin molecules. ICAM2 is expressed widely across many tissues including endothelial cells platelets and leukocytes. Its primary function involves facilitating stable adhesion between leukocytes and endothelial cells which is essential for leukocyte transmigration.
Biological function summary

ICAM2 helps in immune function by serving as an adhesion molecule. It does not form part of a larger protein complex but instead it acts independently to mediate interactions between cells. It contributes to leukocyte rolling and firm adhesion stages necessary for leukocyte extravasation. This function is important in immune surveillance and response to infection allowing immune cells to quickly migrate to sites of inflammation.

Pathways

ICAM2 operates in the leukocyte adhesion and transendothelial migration pathways. It interacts closely with integrins especially LFA-1 present on leukocytes and this interaction supports cell movement during immune responses. It also participates in signaling pathways that activate immune cells further aiding in the regulation of immune cell activity. These interactions make ICAM2 a critical component in immunological communication and signal transduction pathways.

ICAM2 is associated with conditions involving immune dysfunction and inflammation. For instance abnormal expression of ICAM2 has been linked to inflammatory diseases such as atherosclerosis. Additionally it plays a role in the pathology of multiple sclerosis where it might influence the migration of immune cells across the blood-brain barrier. Its relationship with integrins highlights its potential relevance in therapeutic strategies to modulate immune responses in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion on resting cells (PubMed : 10077629, PubMed : 1676048, PubMed : 2497351). Also endothelial blood vessels receptor ligand of dendritic cell (DC) CD209 signaling receptor that mediates triggering transendothelial migration of DC presursors from blood into peripheral tissues and, subsequently, into lymphoid tissues (PubMed : 11017109). Mediates adhesive interactions important for antigen presentation, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance (Probable).
See full target information ICAM2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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