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Rabbit Recombinant Monoclonal ICAM2 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse samples.

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Images

Western blot - Anti-ICAM2 antibody [EPR18231-183] (AB189463), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] (AB189463), expandable thumbnail
  • Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] (AB189463), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] (AB189463), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Mouse
Tested
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/60

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/8000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Alternative names

Intercellular adhesion molecule 2, ICAM-2, Lymphocyte function-associated AG-1 counter-receptor, Icam2, Icam-2

Recommended products

Rabbit Recombinant Monoclonal ICAM2 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse samples.

Alternative names

Intercellular adhesion molecule 2, ICAM-2, Lymphocyte function-associated AG-1 counter-receptor, Icam2, Icam-2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18231-183

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ICAM2 also known as IC2 or ICAM-2 is a cell surface glycoprotein with a molecular mass of approximately 55-65 kDa. It plays an important role in immune response. The protein belongs to the immunoglobulin superfamily and interacts specifically with integrin molecules. ICAM2 is expressed widely across many tissues including endothelial cells platelets and leukocytes. Its primary function involves facilitating stable adhesion between leukocytes and endothelial cells which is essential for leukocyte transmigration.

Biological function summary

ICAM2 helps in immune function by serving as an adhesion molecule. It does not form part of a larger protein complex but instead it acts independently to mediate interactions between cells. It contributes to leukocyte rolling and firm adhesion stages necessary for leukocyte extravasation. This function is important in immune surveillance and response to infection allowing immune cells to quickly migrate to sites of inflammation.

Pathways

ICAM2 operates in the leukocyte adhesion and transendothelial migration pathways. It interacts closely with integrins especially LFA-1 present on leukocytes and this interaction supports cell movement during immune responses. It also participates in signaling pathways that activate immune cells further aiding in the regulation of immune cell activity. These interactions make ICAM2 a critical component in immunological communication and signal transduction pathways.

Associated diseases and disorders

ICAM2 is associated with conditions involving immune dysfunction and inflammation. For instance abnormal expression of ICAM2 has been linked to inflammatory diseases such as atherosclerosis. Additionally it plays a role in the pathology of multiple sclerosis where it might influence the migration of immune cells across the blood-brain barrier. Its relationship with integrins highlights its potential relevance in therapeutic strategies to modulate immune responses in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ICAM2 antibody [EPR18231-183] (ab189463), expandable thumbnail

    Western blot - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 1676048).

    Blocking/Dilution: 5% NFDM/TBST

    Exposure: Lane1: 5 seconds; lanes 2 and 3: 3 minutes

    All lanes: Western blot - Anti-ICAM2 antibody [EPR18231-183] (ab189463) at 1/1000 dilution

    Lane 1: bEnd.3 (mouse brain endothelioma cell line) cell lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] (ab189463), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling ICAM2 with ab189463 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Positive staining on vascular endothelium of mouse cerebral cortex (PMID: 12516546). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] (ab189463), expandable thumbnail

    Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

    ICAM2 was immunoprecipitated from 0.35 mg of bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab189463 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab189463 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: bEnd.3 whole cell lysate 10 μg (Input).

    Lane 2: ab189463 IP in bEnd.3 whole cell lysate (+).

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab189463 in bEnd.3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds

    All lanes: Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

    Predicted band size: 31 kDa

    Observed band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] (ab189463), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] (ab189463)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling ICAM2 with ab189463 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Positive staining on vascular endothelium of mouse spleen (PMID: 12516546). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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