Rabbit Recombinant Monoclonal ICAM2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (AB224531), expandable thumbnail](https://content.abcam.com/products/images/icam2-antibody-epr18231-183-bsa-and-azide-free-ab224531--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125109.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (AB224531)")
![Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (AB224531), expandable thumbnail](https://content.abcam.com/products/images/icam2-antibody-epr18231-183-bsa-and-azide-free-ab224531--immunoprecipitation-img5910.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (AB224531)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (AB224531), expandable thumbnail](https://content.abcam.com/products/images/icam2-antibody-epr18231-183-bsa-and-azide-free-ab224531--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130251.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (AB224531)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Mouse | Tested | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
Alternative names
Intercellular adhesion molecule 2, ICAM-2, Lymphocyte function-associated AG-1 counter-receptor, Icam2, Icam-2
Recommended products
Rabbit Recombinant Monoclonal ICAM2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples.
Alternative names
Intercellular adhesion molecule 2, ICAM-2, Lymphocyte function-associated AG-1 counter-receptor, Icam2, Icam-2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR18231-183
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab224531 is the carrier-free version of Anti-ICAM2 antibody [EPR18231-183] ab189463.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ICAM2 also known as IC2 or ICAM-2 is a cell surface glycoprotein with a molecular mass of approximately 55-65 kDa. It plays an important role in immune response. The protein belongs to the immunoglobulin superfamily and interacts specifically with integrin molecules. ICAM2 is expressed widely across many tissues including endothelial cells platelets and leukocytes. Its primary function involves facilitating stable adhesion between leukocytes and endothelial cells which is essential for leukocyte transmigration.
- Biological function summary
ICAM2 helps in immune function by serving as an adhesion molecule. It does not form part of a larger protein complex but instead it acts independently to mediate interactions between cells. It contributes to leukocyte rolling and firm adhesion stages necessary for leukocyte extravasation. This function is important in immune surveillance and response to infection allowing immune cells to quickly migrate to sites of inflammation.
- Pathways
ICAM2 operates in the leukocyte adhesion and transendothelial migration pathways. It interacts closely with integrins especially LFA-1 present on leukocytes and this interaction supports cell movement during immune responses. It also participates in signaling pathways that activate immune cells further aiding in the regulation of immune cell activity. These interactions make ICAM2 a critical component in immunological communication and signal transduction pathways.
- Associated diseases and disorders
ICAM2 is associated with conditions involving immune dysfunction and inflammation. For instance abnormal expression of ICAM2 has been linked to inflammatory diseases such as atherosclerosis. Additionally it plays a role in the pathology of multiple sclerosis where it might influence the migration of immune cells across the blood-brain barrier. Its relationship with integrins highlights its potential relevance in therapeutic strategies to modulate immune responses in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531), expandable thumbnail](https://content.abcam.com/products/images/icam2-antibody-epr18231-183-bsa-and-azide-free-ab224531--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125109.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling ICAM2 with Anti-ICAM2 antibody [EPR18231-183] ab189463 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Positive staining on vascular endothelium of mouse cerebral cortex (PMID: 12516546). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ICAM2 antibody [EPR18231-183] ab189463).
![Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531), expandable thumbnail](https://content.abcam.com/products/images/icam2-antibody-epr18231-183-bsa-and-azide-free-ab224531--immunoprecipitation-img5910.jpg "Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531)")
Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531)
ICAM2 was immunoprecipitated from 0.35 mg of bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with Anti-ICAM2 antibody [EPR18231-183] ab189463 at 1/60 dilution. Western blot was performed from the immunoprecipitate using Anti-ICAM2 antibody [EPR18231-183] ab189463 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Lane 1: bEnd.3 whole cell lysate 10 μg (Input).
Lane 2: Anti-ICAM2 antibody [EPR18231-183] ab189463 IP in bEnd.3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ICAM2 antibody [EPR18231-183] ab189463 in bEnd.3 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 30 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ICAM2 antibody [EPR18231-183] ab189463).
All lanes: Immunoprecipitation - Anti-ICAM2 antibody [EPR18231-183] (Anti-ICAM2 antibody [EPR18231-183] ab189463)
Predicted band size: 31 kDa
Observed band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531), expandable thumbnail](https://content.abcam.com/products/images/icam2-antibody-epr18231-183-bsa-and-azide-free-ab224531--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130251.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR18231-183] - BSA and Azide free (ab224531)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling ICAM2 with Anti-ICAM2 antibody [EPR18231-183] ab189463 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Positive staining on vascular endothelium of mouse spleen (PMID: 12516546). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ICAM2 antibody [EPR18231-183] ab189463).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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