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Rabbit Recombinant Monoclonal ICAM2 antibody. Suitable for WB, IHC-P and reacts with Human samples.


Images

Western blot - Anti-ICAM2 antibody [EPR19114-113] (AB197570), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR19114-113] (AB197570), expandable thumbnail
  • Western blot - Anti-ICAM2 antibody [EPR19114-113] (AB197570), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR19114-113] (AB197570), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Alternative names

Intercellular adhesion molecule 2, ICAM-2, ICAM2

Recommended products

Rabbit Recombinant Monoclonal ICAM2 antibody. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19114-113

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ICAM2 antibody [EPR19114-113] (ab197570), expandable thumbnail

    Western blot - Anti-ICAM2 antibody [EPR19114-113] (ab197570)

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 1676048).

    All lanes: Western blot - Anti-ICAM2 antibody [EPR19114-113] (ab197570) at 1/5000 dilution

    All lanes: Jurkat (human T cell leukemia T lymphocyte) lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 31 kDa

    Observed band size: 60 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR19114-113] (ab197570), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR19114-113] (ab197570)

    Immunohistochemical analysis of paraffin-embedded human lung tissue labeling ICAM2 with ab197570 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on vascular endothelium of human lung (PMID: 1676048) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-ICAM2 antibody [EPR19114-113] (ab197570), expandable thumbnail

    Western blot - Anti-ICAM2 antibody [EPR19114-113] (ab197570)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ICAM2 antibody [EPR19114-113] (ab197570) at 1/100 dilution

    Lane 1: Human fetal kidney lysate at 10 µg

    Lane 2: Human fetal spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 31 kDa

    Observed band size: 60 kDa

    Exposure time: 20s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR19114-113] (ab197570), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM2 antibody [EPR19114-113] (ab197570)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling ICAM2 with ab197570 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on vascular endothelium of human spleen (PMID: 1676048) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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