JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB288535

Anti-ICAM3 antibody [B-R1] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal ICAM3 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD50, Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, ICAM3

1 Images
Flow Cytometry - Anti-ICAM3 antibody [B-R1] - BSA and Azide free (AB288535)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ICAM3 antibody [B-R1] - BSA and Azide free (AB288535)

Flow cytometry overlay histogram of human lymphocytes labelling ICAM3 with ab288535 at 1/2 – 1/256 dilution (black, right) compared with an isotype control (grey, left). GAM-FITC at 1/800 dilution was used as the secondary antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-R1

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

style
left-column

Complementary Products

Assay Kits

AB275097

Human ICAM3 ELISA Kit (CD50)

Recombinant|SimpleStep

Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (AB275097)

  • 0
  • 0
Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

  • 5
  • 18
Primary Antibodies

AB64693

Anti-Mannose Receptor antibody

BOND RX™ Validated

Western blot - Anti-Mannose Receptor antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB254222

Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (AB254222)

  • 5
  • 1
Primary Antibodies

AB208670

Anti-Myeloperoxidase antibody [EPR20257]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

  • 5
  • 7
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2 - 1/256", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from cell culture supernatant
Storage buffer
pH: 7.3 - 7.5 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM-3 also known as CD50 or Intercellular Adhesion Molecule 3 is a cell surface glycoprotein. It weighs approximately 120 kilodaltons. ICAM-3 is expressed majorly on leukocytes including lymphocytes monocytes and granulocytes. It is a member of the immunoglobulin superfamily that participates in cell-cell interaction particularly in immune response. By engaging with integrin receptors mainly LFA-1 ICAM-3 plays a role in leukocyte adhesion and signaling.
Biological function summary

ICAM-3 contributes to the immune system’s functionality by mediating intercellular adhesion and cellular signaling. It acts as a ligand for the integrin LFA-1 found on leukocytes which facilitates the initial immune response. ICAM-3 is not a part of a larger complex but works closely with LFA-1 in homing and recruitment of immune cells to inflammation sites. Its interaction with LFA-1 also supports the formation of the immunological synapse an important step in T-cell activation and immune signaling.

Pathways

ICAM-3 contributes to both the immune response and leukocyte extravasation pathways. The interaction with LFA-1 places it within the context of immune cell signaling pathways impacting integrin-mediated signaling. Another pathway involving ICAM-3 is the cell adhesion pathway which is essential for maintaining proper immune surveillance and response. It collaborates with proteins like ICAM-1 in these pathways to ensure effective immune activity.

ICAM-3 is associated with conditions such as inflammatory diseases and certain autoimmune disorders. Its expression levels and binding interactions can affect the severity and progression of such diseases. In autoimmune diseases ICAM-3 interaction with LFA-1 might contribute to the aberrant immune activation observed. Additionally studies have highlighted its role in infections where viruses exploit ICAM proteins to enter or spread showing its importance in pathogenesis-related conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring apoptotic neutrophil phagocytosis by macrophages (PubMed : 1448173, PubMed : 1448174, PubMed : 23775590, PubMed : 8325327, PubMed : 8459213). Is also a ligand for integrin ITGAD/ITGB2 (PubMed : 8777714). Ligand of CD209 on dendritic cells (DC) through an integrin-independent mechanism that requires Ca(2+) and that forms a first contact between DC and resting T cell, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells (PubMed : 10721994).
See full target information ICAM3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com