JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB10804

Anti-ICAM3 antibody [ICAM3.1]

Be the first to review this product! Submit a review

|

(4 Publications)

Mouse Monoclonal ICAM3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ICAM3.

View Alternative Names

CD50, Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, ICAM3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (AB10804)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (AB10804)

ab10804 staining Human malignant stomach. Staining is localised to the membrane.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ICAM3.1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ICAM3. The exact immunogen used to generate this antibody is proprietary information.

P32942

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM-3 also known as CD50 or Intercellular Adhesion Molecule 3 is a cell surface glycoprotein. It weighs approximately 120 kilodaltons. ICAM-3 is expressed majorly on leukocytes including lymphocytes monocytes and granulocytes. It is a member of the immunoglobulin superfamily that participates in cell-cell interaction particularly in immune response. By engaging with integrin receptors mainly LFA-1 ICAM-3 plays a role in leukocyte adhesion and signaling.
Biological function summary

ICAM-3 contributes to the immune system’s functionality by mediating intercellular adhesion and cellular signaling. It acts as a ligand for the integrin LFA-1 found on leukocytes which facilitates the initial immune response. ICAM-3 is not a part of a larger complex but works closely with LFA-1 in homing and recruitment of immune cells to inflammation sites. Its interaction with LFA-1 also supports the formation of the immunological synapse an important step in T-cell activation and immune signaling.

Pathways

ICAM-3 contributes to both the immune response and leukocyte extravasation pathways. The interaction with LFA-1 places it within the context of immune cell signaling pathways impacting integrin-mediated signaling. Another pathway involving ICAM-3 is the cell adhesion pathway which is essential for maintaining proper immune surveillance and response. It collaborates with proteins like ICAM-1 in these pathways to ensure effective immune activity.

ICAM-3 is associated with conditions such as inflammatory diseases and certain autoimmune disorders. Its expression levels and binding interactions can affect the severity and progression of such diseases. In autoimmune diseases ICAM-3 interaction with LFA-1 might contribute to the aberrant immune activation observed. Additionally studies have highlighted its role in infections where viruses exploit ICAM proteins to enter or spread showing its importance in pathogenesis-related conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed : 1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590).
See full target information ICAM3
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of asthma and allergy 14:585-593 PubMed34079296

2021

Cell Adhesion Molecules are Upregulated and May Drive Inflammation in Chronic Rhinosinusitis with Nasal Polyposis.

Applications

Unspecified application

Species

Unspecified reactive species

Brennan J Blight,Amarbir S Gill,Jorgen S Sumsion,Chelsea E Pollard,Shaelene Ashby,Gretchen M Oakley,Jeremiah A Alt,Abigail Pulsipher

Molecular therapy : the journal of the American Society of Gene Therapy 22:149-59 PubMed23985698

2013

Exploiting human CD34+ stem cell-conditioned medium for tissue repair.

Applications

Unspecified application

Species

Unspecified reactive species

Paul J Mintz,Kai-Wen Huang,Vikash Reebye,Georgios Nteliopoulos,Hong-Shiee Lai,Pal Sætrom,Noriyuki Kasahara,Steen Jensen,Madhava Pai,Myrtle Y A Gordon,Stephen B Marley,Rosemary Behan,Duncan R Spalding,Abdelali Haoudi,Mohamed M Emara,Joanna Nicholls,John J Rossi,Nagy A Habib

The Journal of cell biology 190:553-63 PubMed20733052

2010

Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration.

Applications

ICC/IF

Species

Human

Sarah J Heasman,Leo M Carlin,Susan Cox,Tony Ng,Anne J Ridley

The Journal of cell biology 176:863-75 PubMed17353362

2007

The Par polarity complex regulates Rap1- and chemokine-induced T cell polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Audrey Gérard,Alexander E E Mertens,Rob A van der Kammen,John G Collard
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB239270

Alexa Fluor® 488 Anti-ICAM3 antibody [MEM-171]

Flow Cytometry - Alexa Fluor® 488 Anti-ICAM3 antibody [MEM-171] (AB239270)

  • 0
  • 0
Primary Antibodies

AB108618

Anti-ICAM3 antibody [EPR3995]

RabMAb|Recombinant

Western blot - Anti-ICAM3 antibody [EPR3995] (AB108618)

  • 0
  • 0
Primary Antibodies

AB109405

Anti-ICAM3 antibody [EPR3994-123]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [EPR3994-123] (AB109405)

  • 0
  • 0

Complementary Products

Assay Kits

AB275097

Human ICAM3 ELISA Kit (CD50)

Recombinant|SimpleStep

Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (AB275097)

  • 0
  • 0
Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

  • 5
  • 18
Primary Antibodies

AB64693

Anti-Mannose Receptor antibody

BOND RX™ Validated

Western blot - Anti-Mannose Receptor antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB254222

Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (AB254222)

  • 5
  • 1
Primary Antibodies

AB208670

Anti-Myeloperoxidase antibody [EPR20257]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

  • 5
  • 7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com