Rabbit Recombinant Monoclonal ICAM5 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401354.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)")
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401353.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)")
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401352.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)")
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401351.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)")
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401350.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | IP | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).
Alternative names
TLCN, TLN, ICAM5, Intercellular adhesion molecule 5, ICAM-5, Telencephalin
Recommended products
Rabbit Recombinant Monoclonal ICAM5 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR25850-86
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ICAM5 also known as Telencephalin is an intercellular adhesion molecule with a molecular mass of approximately 130 kDa. This protein is mainly expressed in the brain specifically in neurons of the telencephalon region. ICAM5 belongs to the immunoglobulin superfamily and plays a role in cell-cell adhesion. It does not demonstrate tight adherence like other ICAMs which suggests its specific function might differ from other ICAM family members.
- Biological function summary
ICAM5 participates in neuron-neuron adhesion processes. It plays a significant role in the development and maintenance of synaptic structures in the nervous system. ICAM5 does not form a complex with other proteins in its functioning. It contributes to the organization and stabilization of dendritic spines which are important for synaptic plasticity and neural communication. This involvement highlights the importance of ICAM5 in neural development and synaptic function.
- Pathways
ICAM5 integrates into signaling pathways related to neuronal development and synaptic plasticity. It is a part of the adhesion and cell recognition processes in neural systems. Through its involvement ICAM5 associates with pathways like the glutamatergic synapse pathway. Additionally ICAM5 shows a connection with proteins involved in membrane transport and neuronal signaling such as NMDARs (N-methyl-D-aspartate receptors) emphasizing its role in synaptic modulation.
- Associated diseases and disorders
ICAM5 relates to conditions affecting the nervous system such as schizophrenia and Alzheimer's disease. Studies indicate alterations in ICAM5 expression levels in patients with these diseases suggesting a potential impact on synaptic integrity and function. In Alzheimer's disease ICAM5 interacts with pathways involving beta-amyloid peptide accumulation indicating a possible connection with oxidative stress and neurodegenerative processes. In schizophrenia disruptions in ICAM5 associations with neural adhesion molecules could affect synaptic signaling and stability contributing to cognitive deficits.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401354.jpg "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)")
Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat cerebrum. The section was incubated with ab302899 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401353.jpg "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)")
Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)
Immunohistochemical analysis of paraffin-embedded Rat sagittal brain tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat sagittal brain (PMID: 7794412). The section was incubated with ab302899 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401352.jpg "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)")
Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse cerebrum. The section was incubated with ab302899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401351.jpg "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)")
Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)
Immunohistochemical analysis of paraffin-embedded Mouse sagittal brain tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse sagittal brain (PMID: 7794412). The section was incubated with ab302899 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899), expandable thumbnail](https://content.abcam.com/products/images/icam5-antibody-epr25850-86-ab302899--immunohistochemistry-img401350.jpg "Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)")
Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (ab302899)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebrum. The section was incubated with ab302899 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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