Anti-ICAM5 antibody [EPR25850-86]

• IHC

Supplier Data

Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebrum. The section was incubated with ab302899 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)

Immunohistochemical analysis of paraffin-embedded Mouse sagittal brain tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse sagittal brain (PMID : 7794412). The section was incubated with ab302899 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on mouse cerebrum. The section was incubated with ab302899 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat cerebrum. The section was incubated with ab302899 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)

Immunohistochemical analysis of paraffin-embedded Rat sagittal brain tissue labeling ICAM5 with ab302899 at 1/1000 (0.505 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on rat sagittal brain (PMID : 7794412). The section was incubated with ab302899 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-ICAM5 antibody [EPR25850-86] (AB302899)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 17682049)

Low expression : liver, skeletal muscle (PMID : 8995416).

Exposure time : Lane 1-3 : 92 seconds

Lane 4-5 : 180 seconds

All lanes:

Western blot - Anti-ICAM5 antibody [EPR25850-86] (ab302899) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 110 kDa,160 kDa

false

• WB

Supplier Data

Western blot - Anti-ICAM5 antibody [EPR25850-86] (AB302899)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Low expression : liver, skeletal muscle (PMID : 8995416)

Lanes 1 - 6:

Western blot - Anti-ICAM5 antibody [EPR25850-86] (ab302899) at 1/1000 dilution

Lanes 1 - 6:

Western blot - Anti-ICAM5 antibody [EPR25850-86] (BSA and Azide free) (<a href='/en-us/products/primary-antibodies/icam5-antibody-epr25850-86-bsa-and-azide-free-ab302900'>ab302900</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Lane 6:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 160 kDa

false

Exposure time: 10s