Anti-ICB1 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICB1 antibody (AB236975)

Paraffin-embedded human pancreatic cancer tissue stained for ICB1 using ab236975 at 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ICB1 antibody (AB236975)

NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for ICB1 (green) using ab236975 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

• WB

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Western blot - Anti-ICB1 antibody (AB236975)

All lanes:

Western blot - Anti-ICB1 antibody (ab236975) at 1/500 dilution

All lanes:

A549 (Human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 72 kDa

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