AB230543

Anti-ICER antibody

Name: Anti-ICER antibody (ab230543) | Abcam
Brand: Abcam Limited
SKU: AB230543
Price: 605 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal ICER antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human cAMP-responsive element modulator conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

cAMP-responsive element modulator, Inducible cAMP early repressor, ICER, CREM

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICER antibody (AB230543)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICER antibody (AB230543)

Formalin-fixed, paraffin-embedded human brain (cerebellum) tissue stained for ICER using ab230543 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICER antibody (AB230543)

Formalin-fixed, paraffin-embedded human testis tissue stained for ICER using ab230543 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ICER antibody (AB230543)

All lanes:

Western blot - Anti-ICER antibody (ab230543) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human cAMP-responsive element modulator conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q03060

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible cAMP early repressor (ICER) also referred to as ICER is a transcriptional repressor that plays a role in regulating gene expression. ICER is one of several products generated from the cAMP-responsive element modulator (CREM) gene through alternative promoter usage. ICER consists of an estimated molecular mass of around 20-22 kDa depending on the variant. It notably expresses in endocrine tissues such as the pituitary gland and testis as well as in immune cells and the brain. The protein operates by binding to cAMP response elements (CRE) within DNA sequences inhibiting the transcription of certain genes.

Biological function summary

This protein influences gene expression regulation during cellular processes such as differentiation and proliferation. ICER lacks independent transcriptional activity and functions through dimerization with other transcription factors. Although not a part of complex machinery like RNA polymerase ICER affects transcriptional repression via interactions with partners involved in the CREB family. It serves in maintaining cellular homeostasis especially during feedback regulation of genes activated by cAMP signaling.

Pathways

ICER impacts intracellular signaling by being involved in the cAMP signaling pathway and has roles in regulating cellular responses to hormonal stimuli. This pathway affects various physiological systems including the cardiovascular and nervous systems. ICER often interacts with CREB (cAMP-response element-binding protein) within these pathways. CREB and related proteins modulate gene transcription in response to hormonal changes linking ICER's function to broader signaling networks.

ICER relates to cardiac hypertrophy and depression where dysregulated cAMP signaling contributes to pathogenesis. In cardiac hypertrophy ICER disrupts normal gene expression patterns within the heart possibly linked with CREB signaling disruptions. In depression altered ICER expression in brain tissue affects responses to neurotransmitter signaling changes also linking back to CREB and other transcriptional regulators. Understanding ICER's roles in these conditions helps grasp how it influences disease states through transcriptional control.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation (PubMed : 10373550).. Isoform 6. May play a role in the regulation of the circadian clock : acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter.

See full target information cAMP-responsive element modulator

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:4740 PubMed38834545

2024

Burkholderia pseudomallei BipD modulates host mitophagy to evade killing.

Applications

Unspecified application

Species

Unspecified reactive species

Dongqi Nan,Chenglong Rao,Zhiheng Tang,Wenbo Yang,Pan Wu,Jiangao Chen,Yupei Xia,Jingmin Yan,Wenzheng Liu,Ziyuan Zhang,Zhiqiang Hu,Hai Chen,Yaling Liao,Xuhu Mao,Xiaoyun Liu,Quanming Zou,Qian Li

International journal of molecular sciences 20: PubMed31684200

2019

Generation of Organized Porcine Testicular Organoids in Solubilized Hydrogels from Decellularized Extracellular Matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Maxime Vermeulen,Federico Del Vento,Marc Kanbar,Sébastien Pyr Dit Ruys,Didier Vertommen,Jonathan Poels,Christine Wyns

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ICER antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICER antibody (AB230543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICER antibody (AB230543)

Western blot - Anti-ICER antibody (AB230543)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise