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AB196964

Anti-ICK antibody

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(2 Publications)

Rabbit Polyclonal ICK antibody. Suitable for WB and reacts with Mouse, African green monkey, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CILK1.

View Alternative Names

ICK, KIAA0936, CILK1, Serine/threonine-protein kinase ICK, Ciliogenesis associated kinase 1, Intestinal cell kinase, Laryngeal cancer kinase 2, MAK-related kinase, hICK, LCK2, MRK

2 Images
Western blot - Anti-ICK antibody (AB196964)
  • WB

Supplier Data

Western blot - Anti-ICK antibody (AB196964)

All lanes:

Western blot - Anti-ICK antibody (ab196964) at 1/500 dilution

Lane 1:

293 cell extrtact with synthesized peptide

Lane 2:

293 cell extrtact

Lane 3:

COS7 cell extrtact

Predicted band size: 71 kDa

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Western blot - Anti-ICK antibody (AB196964)
  • WB

Supplier Data

Western blot - Anti-ICK antibody (AB196964)

All lanes:

Western blot - Anti-ICK antibody (ab196964) at 1/500 dilution

Lane 1:

3T3 cell extract, treated with PBS (10uM, 60mins)

Lane 2:

3T3 cell extract, treated with PBS (10uM, 60mins) with synthesized peptide

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, African green monkey

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CILK1. The exact immunogen used to generate this antibody is proprietary information.

Q9UPZ9

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Alternative Products

Primary Antibodies

AB224220

Anti-ICK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICK antibody (AB224220)

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Complementary Products

Proteins & Peptides

AB101774

Recombinant human ICK protein

Functional Studies - Recombinant human ICK protein (AB101774)

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Primary Antibodies

AB193624

Anti-WWOX (phospho Y33) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWOX (phospho Y33) antibody (AB193624)

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Primary Antibodies

AB76314

Anti-MEK1 (phospho T292) antibody [EPR2365Y]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-MEK1 (phospho T292) antibody [EPR2365Y] (AB76314)

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Primary Antibodies

AB279383

Anti-MAK antibody [OTI1G2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAK antibody [OTI1G2] (AB279383)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab196964 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICK also known as Intestinal Cell Kinase is a protein kinase with a mass of approximately 72 kDa. It has an important role in regulating cell cycle progression and cellular signaling. The protein expresses in various tissues including the testis brain and skin where it participates in diverse cellular processes. ICK activity can influence cell division and can impact mitotic regulators further elucidating its role in cellular functions.
Biological function summary

The Intestinal Cell Kinase involves in the process of cilia development and maintenance. It interacts as part of a larger protein complex associated with the ciliary basal body. Within this complex ICK modulates the assembly and disassembly of cilia exerting significant control over ciliary motility and length. Its activity is necessary for maintaining the structure and function of these organelles which play important roles in signal transduction pathways critical to cell movement and sensory functions.

Pathways

ICK proteins sit prominently in the Wnt signaling pathway and the Hedgehog signaling pathway. ICK phosphorylates several substrates including proteins tied to these pathways such as GLI transcription factors involved in Hedgehog signaling. Alterations in ICK activity can lead to disruptions in these pathways and affect processes like cellular differentiation and tissue homeostasis.

ICK mutations associate with human ciliopathies such as Nephronophthisis-related disorders. The dysfunction of ICK within the signaling pathways may also link to cancer development where disruptions can affect cell cycle control. Proteins such as GLI involved in the Hedgehog pathway and impacted by ICK are important in these disorders highlighting the potential of ICK as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for ciliogenesis (PubMed : 24797473). Phosphorylates KIF3A (By similarity). Involved in the control of ciliary length (PubMed : 24853502). Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (By similarity). May play a key role in the development of multiple organ systems and particularly in cardiac development (By similarity). Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity (By similarity).
See full target information CILK1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:2403-2421 PubMed38725848

2024

Ciliogenesis-associated Kinase 1 Promotes Breast Cancer Cell Proliferation and Chemoresistance via Phosphorylating ERK1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanling He,Xinyuan Zhang,Weijun Pan,Jiebiao Zhang,Weiliang Zhu,Jian Zhang,Jian Shi

Turk gogus kalp damar cerrahisi dergisi 30:574-583 PubMed36605324

2023

Importance of TAK-1 levels in patients with non-small cell lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pelin Sürücü,Yasemin Büyükkarabacak,Yurdanur Süllü,Ahmet Basoğlu
View all publications
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Product promise

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