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AB224220

Anti-ICK antibody

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(1 Publication)

Rabbit Polyclonal ICK antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CILK1 aa 250-450.

View Alternative Names

ICK, KIAA0936, CILK1, Serine/threonine-protein kinase ICK, Ciliogenesis associated kinase 1, Intestinal cell kinase, Laryngeal cancer kinase 2, MAK-related kinase, hICK, LCK2, MRK

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICK antibody (AB224220)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICK antibody (AB224220)

Paraffin-embedded human colon tissue stained for ICK using ab224220 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CILK1 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q9UPZ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICK also known as Intestinal Cell Kinase is a protein kinase with a mass of approximately 72 kDa. It has an important role in regulating cell cycle progression and cellular signaling. The protein expresses in various tissues including the testis brain and skin where it participates in diverse cellular processes. ICK activity can influence cell division and can impact mitotic regulators further elucidating its role in cellular functions.
Biological function summary

The Intestinal Cell Kinase involves in the process of cilia development and maintenance. It interacts as part of a larger protein complex associated with the ciliary basal body. Within this complex ICK modulates the assembly and disassembly of cilia exerting significant control over ciliary motility and length. Its activity is necessary for maintaining the structure and function of these organelles which play important roles in signal transduction pathways critical to cell movement and sensory functions.

Pathways

ICK proteins sit prominently in the Wnt signaling pathway and the Hedgehog signaling pathway. ICK phosphorylates several substrates including proteins tied to these pathways such as GLI transcription factors involved in Hedgehog signaling. Alterations in ICK activity can lead to disruptions in these pathways and affect processes like cellular differentiation and tissue homeostasis.

ICK mutations associate with human ciliopathies such as Nephronophthisis-related disorders. The dysfunction of ICK within the signaling pathways may also link to cancer development where disruptions can affect cell cycle control. Proteins such as GLI involved in the Hedgehog pathway and impacted by ICK are important in these disorders highlighting the potential of ICK as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for ciliogenesis (PubMed : 24797473). Phosphorylates KIF3A (By similarity). Involved in the control of ciliary length (PubMed : 24853502). Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips (By similarity). May play a key role in the development of multiple organ systems and particularly in cardiac development (By similarity). Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity (By similarity).
See full target information CILK1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 369: PubMed32973005

2020

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Sue Im Sim,Alban Ordureau,Lianjie Wei,J Wade Harper,Sichen Shao,Eunyong Park
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