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AB95630

Anti-ICOS antibody [7E.17G9]

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(1 Publication)

Rat Monoclonal ICOS antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Mouse Icos.

View Alternative Names

CD278, Ailim, Icos, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD28 and CTLA-4-like protein, CD28-related protein 1, CCLP, CRP-1

1 Images
Flow Cytometry - Anti-ICOS antibody [7E.17G9] (AB95630)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ICOS antibody [7E.17G9] (AB95630)

Staining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b Isotype Control (open histogram) or 0.06 µg of ab95630 (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7E.17G9

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Nucleic Acid within Mouse Icos. Database link Q9WVS0

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Alternative Products

Primary Antibodies

AB105227

Anti-ICOS antibody [SP98]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (AB105227)

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Primary Antibodies

AB283488

Alexa Fluor® 555 Anti-ICOS antibody [EPR20560]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-ICOS antibody [EPR20560] (AB283488)

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Primary Antibodies

AB224644

Anti-ICOS antibody [EPR20560]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (AB224644)

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Primary Antibodies

AB237562

Anti-ICOS antibody [EPR22181] - BSA and Azide free

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Flow Cytometry - Anti-ICOS antibody [EPR22181] - BSA and Azide free (AB237562)

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Primary Antibodies

AB225577

Anti-ICOS antibody [EPR20560] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] - BSA and Azide free (AB225577)

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Primary Antibodies

AB236875

Anti-ICOS antibody [EPR22181]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-ICOS antibody [EPR22181] (AB236875)

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Primary Antibodies

AB236226

Anti-ICOS antibody [SP98] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] - BSA and Azide free (AB236226)

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Complementary Products

Assay Kits

AB267638

Human ICOS ELISA Kit

Sandwich ELISA - Human ICOS ELISA Kit (AB267638)

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Primary Antibodies

AB52587

Anti-PD1 antibody [NAT105]

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB20034

Anti-FOXP3 antibody [236A/E7]

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Primary Antibodies

AB15580

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.06-1 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>A 100 μl volume is recommended for staining the cell sample.</p><p><a href='/en-us/products/unavailable/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-bsa-and-azide-free-ab18536'>ab18536</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible T-cell costimulator (ICOS) also known as AILIM or CD278 is a member of the CD28 superfamily of proteins. ICOS is a surface receptor protein approximately 60 kDa in mass. This protein is expressed mainly on activated T cells. It does not appear on naive or resting T cells. ICOS plays an important mechanical role in the immune response as its engagement enhances the production of cytokines and supports cell-cell interactions through its binding to the ICOS ligand.
Biological function summary

ICOS plays a critical role in the regulation of immune responses. It acts as a costimulatory signal for T cells supporting their proliferation and differentiation. ICOS helps form part of a complex involving interactions with the ICOS ligand present on antigen-presenting cells. This interaction affects the function and fate of various T cell subsets including T follicular helper cells which are important for antibody production.

Pathways

ICOS is part of the immune signaling pathway. It associates closely with the CD28 signaling pathway enhancing T-cell activation and survival. ICOS interacts with proteins such as the ICOS ligand (ICOSL) on antigen-presenting cells which helps mediate its effects. These pathways are essential in immune system homeostasis and adaptive immune responses where dysfunction can lead to immune-related diseases.

ICOS has implications in conditions such as autoimmune diseases and cancer. Abnormal expression or function of ICOS relates to the development of autoimmune conditions like systemic lupus erythematosus (SLE) where the overactive immune response damages the body's tissues. Additionally ICOS might support tumor progression in certain types of cancers by promoting immune escape. It interacts with regulatory proteins like CTLA-4 and PD-1 in these diseases which are notably involved in immune checkpoint pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed : 11343123). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed : 10657606). Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed : 36754569). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (PubMed : 27693916). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed : 19915142, PubMed : 27693916). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (PubMed : 11343122).
See full target information Icos
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communications 306:819-25 PubMed12821115

2003

Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Masafumi Nakayama,Norihiro Harada,Ko Okumura,Hideo Yagita
View all publications
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