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Rat Monoclonal ICOS antibody. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Recombinant Protein within Mouse Icos.

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Images

Flow Cytometry - Anti-ICOS antibody [7E.17G9] (AB95630), expandable thumbnail

Key facts

Isotype

IgG2b

Host species

Rat

Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.87% Sodium chloride

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Protein within Mouse Icos. The exact immunogen used to generate this antibody is proprietary information. Database link Q9WVS0

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

0.06000-1.00000 µg for 105-108 Cells

Notes

A 100 μl volume is recommended for staining the cell sample.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

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Target data

Function

Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed:11343123). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed:10657606). Acts also as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed:36754569). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (PubMed:27693916). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed:19915142, PubMed:27693916). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (PubMed:11343122).

Alternative names

Ailim, Ailim, Icos, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD28 and CTLA-4-like protein, CD28-related protein 1, CCLP, CRP-1

Recommended products

Rat Monoclonal ICOS antibody. Suitable for Flow Cyt and reacts with Mouse samples. Immunogen corresponding to Recombinant Protein within Mouse Icos.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogens
  • Recombinant Protein within Mouse Icos. The exact immunogen used to generate this antibody is proprietary information. Database link Q9WVS0
Clone number

7E.17G9

Purification technique

Affinity purification Protein G

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Inducible T-cell costimulator (ICOS) also known as AILIM or CD278 is a member of the CD28 superfamily of proteins. ICOS is a surface receptor protein approximately 60 kDa in mass. This protein is expressed mainly on activated T cells. It does not appear on naive or resting T cells. ICOS plays an important mechanical role in the immune response as its engagement enhances the production of cytokines and supports cell-cell interactions through its binding to the ICOS ligand.

Biological function summary

ICOS plays a critical role in the regulation of immune responses. It acts as a costimulatory signal for T cells supporting their proliferation and differentiation. ICOS helps form part of a complex involving interactions with the ICOS ligand present on antigen-presenting cells. This interaction affects the function and fate of various T cell subsets including T follicular helper cells which are important for antibody production.

Pathways

ICOS is part of the immune signaling pathway. It associates closely with the CD28 signaling pathway enhancing T-cell activation and survival. ICOS interacts with proteins such as the ICOS ligand (ICOSL) on antigen-presenting cells which helps mediate its effects. These pathways are essential in immune system homeostasis and adaptive immune responses where dysfunction can lead to immune-related diseases.

Associated diseases and disorders

ICOS has implications in conditions such as autoimmune diseases and cancer. Abnormal expression or function of ICOS relates to the development of autoimmune conditions like systemic lupus erythematosus (SLE) where the overactive immune response damages the body's tissues. Additionally ICOS might support tumor progression in certain types of cancers by promoting immune escape. It interacts with regulatory proteins like CTLA-4 and PD-1 in these diseases which are notably involved in immune checkpoint pathways.

Product promise

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1 product image

  • Flow Cytometry - Anti-ICOS antibody [7E.17G9] (ab95630), expandable thumbnail

    Flow Cytometry - Anti-ICOS antibody [7E.17G9] (ab95630)

    Staining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b Isotype Control (open histogram) or 0.06 µg of ab95630 (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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