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Rabbit Recombinant Monoclonal ICOS antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.


Images

Immunoprecipitation - Anti-ICOS antibody [EPR20560] (AB224644), expandable thumbnail
  • Western blot - Anti-ICOS antibody [EPR20560] (AB224644), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (AB224644), expandable thumbnail
  • Western blot - Anti-ICOS antibody [EPR20560] (AB224644), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (AB224644), expandable thumbnail

Publications

  • Frontiers in immunology 14:11713082023
    Involvement of CD40-CD40L and ICOS-ICOSL in the development of chronic rhinosinusitis by targeting eosinophils.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Aina Zhou,Chenxi Shi,Yuhui Fan,Yushuang Zheng,Jue Wang,Zhichen Liu,Huanxia Xie,Jisheng Liu,Qingqing Jiao
    PubMed 37325657
  • Journal of inflammation research 16:977-9922023
    ICOS/ICOSLG and PD-1 Co-Expression is Associated with the Progression of Colorectal Precancerous- Carcinoma Immune Microenvironment.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yu Zhang,Xue-Li Wang,Jing-Jing Liu,Zhen-Yuan Qian,Zheng-Yang Pan,Ni-Ping Song,Hui-Yan Chen,Wei Zhang,Xin Zhang
    PubMed 36915615

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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    4 products for Alternative Version

      Target data

      Function

      Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).

      Alternative names

      Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, AILIM, ICOS

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      Rabbit Recombinant Monoclonal ICOS antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

      Alternative names

      Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, AILIM, ICOS

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR20560

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      9 product images

      • Immunoprecipitation - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Immunoprecipitation - Anti-ICOS antibody [EPR20560] (ab224644)

        ICOS was immunoprecipitated from 0.35 mg of human tonsil tissue lysate with ab224644 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224644 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

        Lane 1: Human tonsil tissue lysate 10 μg (Input).
        Lane 2: ab224644 IP in human tonsil tissue lysate (+).
        Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab224644 in human tonsil tissue lysate (-).

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 1 second.


        All lanes: Immunoprecipitation - Anti-ICOS antibody [EPR20560] (ab224644)

        Developed using the ECL technique.

        Predicted band size: 22 kDa

        Observed band size: 27-29 kDa

      • Western blot - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Western blot - Anti-ICOS antibody [EPR20560] (ab224644)

        Blocking and dilution buffer: 5% NFDM/TBST

        All lanes: Western blot - Anti-ICOS antibody [EPR20560] (ab224644) at 1/1000 dilution

        All lanes: His-tagged human ICOS (aa21-140-GGGGS-aa162-199) recombinant protein, 10 ng

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Developed using the ECL technique.

        Predicted band size: 22 kDa

        Observed band size: 17 kDa

        Exposure time: 1s

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644)

        Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ICOS with ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in a population of lymphoid cells of human tonsil (PMID: 20207847), (magnification x200) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

      • Western blot - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Western blot - Anti-ICOS antibody [EPR20560] (ab224644)

        Blocking and dilution buffer: 5% NFDM/TBST.

        The expression profile observed is consistent with what has been described in the literature (PMID: 11169414).

        All lanes: Western blot - Anti-ICOS antibody [EPR20560] (ab224644) at 1/1000 dilution

        Lane 1: Human tymus tissue lysate at 20 µg

        Lane 2: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Developed using the ECL technique.

        Predicted band size: 22 kDa

        Observed band size: 27-29 kDa

        Exposure time: 10s

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644)

        Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling ICOS with ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in tumor infiltrating lymphoid cells of human cervix cancer (PMID: 23802069) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

      • Western blot - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Western blot - Anti-ICOS antibody [EPR20560] (ab224644)

        Blocking and dilution buffer: 5% NFDM/TBST.

        When untreated two distinct bands of 27kDa and 29kDa were observed. Treatment with PNGase F resulted in a single band with a molecular of 22kDa.

        The expression profile observed is consistent with what has been described in the literature (PMID: 11169414 ).

        All lanes: Western blot - Anti-ICOS antibody [EPR20560] (ab224644) at 1/1000 dilution

        Lane 1: Untreated human tonsil tissue lysate at 20 µg

        Lane 2: Human tonsil tissue lysate treated with PNGase F at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Developed using the ECL technique.

        Predicted band size: 22 kDa

        Observed band size: 22 kDa, 27-29 kDa

        Exposure time: 10s

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling ICOS with ab224644 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in a population of lymphoid cells of mouse spleen is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644)

        Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling ICOS with ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in a population of lymphoid cells of human stomach (magnification x200) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (ab224644)

        Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ICOS with ab224644 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in a population of lymphoid cells of rat spleen is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

        Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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      Product protocols

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