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Rabbit Recombinant Monoclonal ICOS Ligand/ICOSL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (AB209262), expandable thumbnail
  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (AB209262), expandable thumbnail
  • Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (AB209262), expandable thumbnail
  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (AB209262), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/40

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed:11007762, PubMed:11023515, PubMed:30498080). Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP) (PubMed:30498080).

Alternative names

B7H2, B7RP1, ICOSL, KIAA0653, B7H2, ICOSLG, KIAA0653, B7RP1, ICOSL, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, B7-H2, B7RP-1

Recommended products

Rabbit Recombinant Monoclonal ICOS Ligand/ICOSL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20240

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262), expandable thumbnail

    Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/20000 dilution

    All lanes: His-tagged recombinant human ICOS ligand/ICOSL protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa

    Observed band size: 50 kDa

    Exposure time: 10s

    This data was developed using ab209262, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262), expandable thumbnail

    Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    ICOS ligand/ICOSL is highly glycosylated (PMID 11983910).

    Observed band sizes: 60 kDa (glycosylated form); 35 kDa (non-glycosylated form).

    All lanes: Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution

    Lane 1: Untreated JAR (Human placenta choriocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: JAR whole cell lysate treated with PNGase F at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Exposure time: 30s

    This data was developed using ab209262, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    ICOS ligand/ICOSL is highly glycosylated (PMID 11983910).

    Observed band sizes: 60 kDa (glycosylated form); 35 kDa (non-glycosylated form).

  • Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262), expandable thumbnail

    Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)

    ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg of JAR (Human placenta choriocarcinoma cell line) whole cell lysate with ab209262 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209262 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: JAR whole cell lysate, 10 μg (Input).

    Lane 2: ab209262 IP in JAR whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab209262 in JAR whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)

    Predicted band size: 33 kDa

    Observed band size: 60 kDa

  • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262), expandable thumbnail

    Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID 11983910).

    All lanes: Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution

    Lane 1: Untreated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

    Lane 2: HUVEC whole cell lysate treated with tumor necrosis factor-α (TNF-alpha, Recombinant human TNF alpha protein ab9642) 200 unit/ml for 24 hours at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 60 kDa

    Exposure time: 30s

    This data was developed using ab209262, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID: 11983910).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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