AB230946

Anti-ICOS Ligand/ICOSL antibody [EPR20786]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ICOS Ligand/ICOSL antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD275, B7H2, B7RP1, ICOSL, KIAA0653, ICOSLG, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, B7-H2, B7RP-1

4 Images

Supplier Data

Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg JAR (human placenta choriocarcinoma cell line) whole cell lysate with ab230946 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230946 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : JAR (human placenta choriocarcinoma cell line) whole cell lysate 10 μg (Input).
Lane 2 : ab230946 IP in JAR whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230946 in JAR whole cell lysate (-).

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946)

Predicted band size: 33 kDa

Observed band size: 60 kDa

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Supplier Data

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure times :

Lane 1 : 15 seconds.

Lane 2 : 84 seconds.

ICOS ligand/ICOSL is highly glycosylated (PMID11983910).

All lanes:

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946) at 1/1000 dilution

Lane 1:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg

Lane 2:

JAR (human placenta choriocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 60 kDa

false

Supplier Data

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 58 seconds.

The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID 11983910，PMID16049332).

All lanes:

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946) at 1/1000 dilution

Lane 1:

Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 10 µg

Lane 2:

HUVEC whole cell lysate treated with tumor necrosis factor (TNF-alpha, <a href='/en-us/products/proteins-peptides/recombinant-human-tnf-alpha-protein-ab9642'>ab9642</a>) 200 unit/ml for 24 hours at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 60 kDa

false

Supplier Data

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (ab230946) at 1/1000 dilution

All lanes:

His-tagged recombinant human ICOS ligand/ICOSL protein (aa19-258) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa

Observed band size: 55 kDa

false

Carrier free

Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20786

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ICOS Ligand also known as ICOSL B7-H2 and HK53 is a critical component in immune system function. This protein with a molecular mass of approximately 65 kDa is expressed on professional antigen-presenting cells such as dendritic cells macrophages and B cells. It plays a significant mechanical role in the immune response by engaging with its counter receptor the ICOS protein present on the surface of activated T cells. This interaction supports the regulation of T cell activity influencing proliferation and cytokine production.

Biological function summary

ICOSL contributes to the modulation of immune responses. By forming a complex with the active ICOS protein ICOSL is involved in the co-stimulation of T cells. This process is important for the development of T-cell-dependent immune responses and helps in promoting the survival and differentiation of T cells. Together the ICOS ligand and ICOS protein create a dynamic interaction that balances immune activation and tolerance which is essential for maintaining immune homeostasis.

Pathways

The ICOS-ICOSL interaction plays an important role in the T-cell receptor (TCR) signaling pathway. This pathway is fundamental for the activation of T cells and subsequent immune response. ICOSL also connects to the PI3K/Akt pathway where it impacts cell survival and proliferation. ICOS and the PI3K/Akt pathway work together to influence various aspects of immune regulation and adaptive immunity.

ICOSL has a notable impact on autoimmune diseases and cancer. Its function in immune modulation when dysregulated can contribute to autoimmune conditions such as systemic lupus erythematosus. In this context the abnormal activation of the ICOS protein may exaggerate immune responses leading to pathology. In cancer high expression of ICOSL can support tumor progression by affecting the immune surveillance capability. These associations reveal ICOSL’s role in balancing immune activation and inhibition which is important in the context of disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed : 11007762, PubMed : 11023515, PubMed : 30498080). Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP) (PubMed : 30498080).

See full target information ICOSLG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Microorganisms 11: PubMed36838330

2023

SLAMF7/STAT6 Pathway Inhibits Innate Immune Response in Late-Stage Human Keratitis: A Comparative Transcriptome Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Wei,Yuheng Zhang,Qiankun Chen,Xizhan Xu,Zhiqiang Pan,Zi-Bing Jin,Qingfeng Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ICOS Ligand/ICOSL antibody [EPR20786]

Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise