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AB325246

Anti-ICOS Ligand/ICOSL antibody [EPR30369-543] - BSA and Azide free

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Rabbit Recombinant Monoclonal ICOS Ligand/ICOSL antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD275, B7h, B7h2, B7rp1, Icosl, Icoslg, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, LICOS, B7-H2, B7RP-1

3 Images
Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR30369-543] - BSA and Azide free (AB325246)
  • Flow Cyt

Lab

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR30369-543] - BSA and Azide free (AB325246)

This data was developed using ab325235, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) and A20 (mouse reticulum sarcoma B lymphocyte, Right) labelling ICOS Ligand/ICOSL with ab325235 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : EL4.
Gated on viable cells.

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR30369-543] - BSA and Azide free (AB325246)
  • Flow Cyt

Lab

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR30369-543] - BSA and Azide free (AB325246)

This data was developed using ab325235, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse bone marrow treated with 20ng/ml GM-CSF for 13 days (Lower left and right) and Untreated mouse bone marrow (Upper left and right) labelling ICOS Ligand/ICOSL with ab325235 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD11c conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR30369-543] - BSA and Azide free (AB325246)
  • Flow Cyt

Lab

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR30369-543] - BSA and Azide free (AB325246)

This data was developed using ab325235, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell labelling ICOS Ligand/ICOSL with ab325235 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD45R/B220 conjugated to Brilliant Violet 421.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30369-543

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab325246 is the carrier-free version of ab325235

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for the T-cell-specific cell surface receptor ICOS (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; also induces B-cell proliferation and differentiation into plasma cells (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (Probable). During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.
See full target information Icoslg
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com