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AB324345

Anti-ICOS Ligand/ICOSL antibody [EPR30369-585]

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Rabbit Recombinant Monoclonal ICOS Ligand/ICOSL antibody. Suitable for WB and reacts with Mouse samples.

View Alternative Names

CD275, B7h, B7h2, B7rp1, Icosl, Icoslg, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, LICOS, B7-H2, B7RP-1

2 Images
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] (AB324345)
  • WB

Supplier Data

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] (AB324345)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

The blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] (ab324345) at 1/1000 dilution

Lane 1:

Untreated A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

A20 treated with Peptide:N-glycosidase F (PNGase F) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,35 kDa,36 kDa

true

Exposure time: 114s

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] (AB324345)
  • WB

Supplier Data

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] (AB324345)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain (PMID : 10549624).

Negative control : EL4 (PMID : 21958057).

The bands beneath the target band are likely to be degradation products.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1, 2 : 158 seconds Lane 3, 4 : 59 seconds Lane 5, 6 : 26 seconds

All lanes:

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] (ab324345) at 1/1000 dilution

Lane 1:

Mouse lymph node tissue lysate at 50 µg

Lane 2:

Mouse kidney tissue lysate at 50 µg

Lane 3:

Mouse spleen tissue lysate at 50 µg

Lane 4:

Mouse brain tissue lysate at 50 µg

Lane 5:

A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 50 µg

Lane 6:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,36 kDa

false

  • Carrier free

    Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30369-585

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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AB272475

Human ICOS Ligand ELISA Kit (B7-H2)

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View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for the T-cell-specific cell surface receptor ICOS (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (Probable). During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.
See full target information Icoslg
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com