Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] - BSA and Azide free (AB324356)

This data was developed using ab324345, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

The blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] (<a href='/en-us/products/primary-antibodies/icos-ligand-icosl-antibody-epr30369-585-ab324345'>ab324345</a>) at 1/1000 dilution

Lane 1:

Untreated A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

A20 treated with Peptide:N-glycosidase F (PNGase F) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,35 kDa,36 kDa

true

Exposure time: 114s

• WB

Supplier Data

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] - BSA and Azide free (AB324356)

This data was developed using ab324345, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain (PMID : 10549624).

Negative control : EL4 (PMID : 21958057).

The bands beneath the target band are likely to be degradation products.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1, 2 : 158 seconds Lane 3, 4 : 59 seconds Lane 5, 6 : 26 seconds

All lanes:

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR30369-585] (<a href='/en-us/products/primary-antibodies/icos-ligand-icosl-antibody-epr30369-585-ab324345'>ab324345</a>) at 1/1000 dilution

Lane 1:

Mouse lymph node tissue lysate at 50 µg

Lane 2:

Mouse kidney tissue lysate at 50 µg

Lane 3:

Mouse spleen tissue lysate at 50 µg

Lane 4:

Mouse brain tissue lysate at 50 µg

Lane 5:

A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 50 µg

Lane 6:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,36 kDa

false