JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB245678

Anti-ICOS Ligand/ICOSL antibody [HK53]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ICOS Ligand/ICOSL antibody. Suitable for ICC/IF and reacts with Mouse samples. Immunogen corresponding to Cell preparation containing Icoslg protein.

View Alternative Names

CD275, B7h, B7h2, B7rp1, Icosl, Icoslg, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, LICOS, B7-H2, B7RP-1

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [HK53] (AB245678)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [HK53] (AB245678)

PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling ICOS Ligand/ICOSL using ab245678 at 10 μg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 μg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HK53

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

Cell preparation containing Icoslg protein. The exact immunogen used to generate this antibody is proprietary information.

Q9JHJ8

Specificity

This antibody specifically binds to mouse ICOSL and competes with binding of ICOS.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ICOS Ligand also known as ICOSL B7-H2 and HK53 is a critical component in immune system function. This protein with a molecular mass of approximately 65 kDa is expressed on professional antigen-presenting cells such as dendritic cells macrophages and B cells. It plays a significant mechanical role in the immune response by engaging with its counter receptor the ICOS protein present on the surface of activated T cells. This interaction supports the regulation of T cell activity influencing proliferation and cytokine production.
Biological function summary

ICOSL contributes to the modulation of immune responses. By forming a complex with the active ICOS protein ICOSL is involved in the co-stimulation of T cells. This process is important for the development of T-cell-dependent immune responses and helps in promoting the survival and differentiation of T cells. Together the ICOS ligand and ICOS protein create a dynamic interaction that balances immune activation and tolerance which is essential for maintaining immune homeostasis.

Pathways

The ICOS-ICOSL interaction plays an important role in the T-cell receptor (TCR) signaling pathway. This pathway is fundamental for the activation of T cells and subsequent immune response. ICOSL also connects to the PI3K/Akt pathway where it impacts cell survival and proliferation. ICOS and the PI3K/Akt pathway work together to influence various aspects of immune regulation and adaptive immunity.

ICOSL has a notable impact on autoimmune diseases and cancer. Its function in immune modulation when dysregulated can contribute to autoimmune conditions such as systemic lupus erythematosus. In this context the abnormal activation of the ICOS protein may exaggerate immune responses leading to pathology. In cancer high expression of ICOSL can support tumor progression by affecting the immune surveillance capability. These associations reveal ICOSL’s role in balancing immune activation and inhibition which is important in the context of disease.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ligand for the T-cell-specific cell surface receptor ICOS (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells (PubMed : 10549624, PubMed : 10617205, PubMed : 10657606). Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (Probable). During pregnancy, may function to skew the cytokine of maternal T-cells toward immunoprotective Th2 phenotype.
See full target information Icoslg
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB244428

Anti-ICOS Ligand/ICOSL antibody

Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody (AB244428)

  • 0
  • 0
Primary Antibodies

AB209262

Anti-ICOS Ligand/ICOSL antibody [EPR20240]

RabMAb|Recombinant|Trial Size

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (AB209262)

  • 0
  • 0
Primary Antibodies

AB239524

Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20786] - BSA and Azide free (AB239524)

  • 0
  • 0
Primary Antibodies

AB230946

Anti-ICOS Ligand/ICOSL antibody [EPR20786]

RabMAb|Recombinant

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20786] (AB230946)

  • 0
  • 0
Primary Antibodies

AB124972

Anti-ICOS Ligand/ICOSL antibody [EPR6071]

RabMAb|Recombinant

Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (AB124972)

  • 0
  • 0

Complementary Products

Assay Kits

AB272475

Human ICOS Ligand ELISA Kit (B7-H2)

Recombinant|SimpleStep

Sandwich ELISA - Human ICOS Ligand ELISA Kit (B7-H2) (AB272475)

  • 0
  • 0
Primary Antibodies

AB188181

Anti-CD40 antibody [EPR18005-35]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-35] (AB188181)

  • 0
  • 0
Primary Antibodies

AB224644

Anti-ICOS antibody [EPR20560]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [EPR20560] (AB224644)

  • 1
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com