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Mouse Monoclonal ICOS Ligand/ICOSL antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ICOSLG aa 1-150.

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Images

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human ICOSLG aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link O75144

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

1-2ug/ml for 30 min at RT.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1-2 µg for 106 Cells

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

6 products for Alternative Product

Target data

Function

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed:11007762, PubMed:11023515, PubMed:30498080). Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP) (PubMed:30498080).

Alternative names

B7H2, B7RP1, ICOSL, KIAA0653, B7H2, ICOSLG, KIAA0653, B7RP1, ICOSL, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, B7-H2, B7RP-1

Recommended products

Mouse Monoclonal ICOS Ligand/ICOSL antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ICOSLG aa 1-150.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human ICOSLG aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link O75144
Carrier free

Yes

Clone number

ICOSL/3111

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...
Purification notes

Purified from Bioreactor Concentrate.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab259280 is the carrier-free version of Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ICOS Ligand also known as ICOSL B7-H2 and HK53 is a critical component in immune system function. This protein with a molecular mass of approximately 65 kDa is expressed on professional antigen-presenting cells such as dendritic cells macrophages and B cells. It plays a significant mechanical role in the immune response by engaging with its counter receptor the ICOS protein present on the surface of activated T cells. This interaction supports the regulation of T cell activity influencing proliferation and cytokine production.

Biological function summary

ICOSL contributes to the modulation of immune responses. By forming a complex with the active ICOS protein ICOSL is involved in the co-stimulation of T cells. This process is important for the development of T-cell-dependent immune responses and helps in promoting the survival and differentiation of T cells. Together the ICOS ligand and ICOS protein create a dynamic interaction that balances immune activation and tolerance which is essential for maintaining immune homeostasis.

Pathways

The ICOS-ICOSL interaction plays an important role in the T-cell receptor (TCR) signaling pathway. This pathway is fundamental for the activation of T cells and subsequent immune response. ICOSL also connects to the PI3K/Akt pathway where it impacts cell survival and proliferation. ICOS and the PI3K/Akt pathway work together to influence various aspects of immune regulation and adaptive immunity.

Associated diseases and disorders

ICOSL has a notable impact on autoimmune diseases and cancer. Its function in immune modulation when dysregulated can contribute to autoimmune conditions such as systemic lupus erythematosus. In this context the abnormal activation of the ICOS protein may exaggerate immune responses leading to pathology. In cancer high expression of ICOSL can support tumor progression by affecting the immune surveillance capability. These associations reveal ICOSL’s role in balancing immune activation and inhibition which is important in the context of disease.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280), expandable thumbnail

    Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280)

    Flow Cytometric analysis of U937 cells staining ICOSL with Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321, followed by a goat anti-Mouse IgG-CF488 secondary antibody (blue). Isotype control (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma staining ICOS Ligand/ICOSL with Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321 at 2μg/ml for 30 minutes at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321).

  • Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280)

    Immunocytochemistry/Immunofluorescent analysis of U937 cells staining ICOS ligand/ICOSL with Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321 at 2μg/ml, followed by goat anti-Mouse IgG conjugated to CF488 secondary antibody (green)/ Nuclei counterstained with Reddot (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma staining ICOS Ligand/ICOSL with Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321 at 2μg/ml for 30 minutes at room temperature.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] ab257321).

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Product protocols

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