AB259280

Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free

Name: Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (ab259280) | Abcam
Brand: Abcam Limited
SKU: AB259280
Price: 915 USD
Availability: InStock

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Mouse Monoclonal ICOS Ligand/ICOSL antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ICOSLG aa 1-150.

View Alternative Names

CD275, B7H2, B7RP1, ICOSL, KIAA0653, ICOSLG, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, B7-H2, B7RP-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma staining ICOS Ligand/ICOSL with ab257321 at 2μg/ml for 30 minutes at room temperature.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257321).

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Flow Cytometric analysis of U937 cells staining ICOSL with ab257321, followed by a goat anti-Mouse IgG-CF488 secondary antibody (blue). Isotype control (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257321).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma staining ICOS Ligand/ICOSL with ab257321 at 2μg/ml for 30 minutes at room temperature.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257321).

Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Immunocytochemistry/Immunofluorescent analysis of U937 cells staining ICOS ligand/ICOSL with ab257321 at 2μg/ml, followed by goat anti-Mouse IgG conjugated to CF488 secondary antibody (green)/ Nuclei counterstained with Reddot (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab257321).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ICOSL/3111

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ICOSLG aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O75144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "1-2ug/ml for 30 min at RT.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

ab259280 is the carrier-free version of ab257321.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor Concentrate.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ICOS Ligand also known as ICOSL B7-H2 and HK53 is a critical component in immune system function. This protein with a molecular mass of approximately 65 kDa is expressed on professional antigen-presenting cells such as dendritic cells macrophages and B cells. It plays a significant mechanical role in the immune response by engaging with its counter receptor the ICOS protein present on the surface of activated T cells. This interaction supports the regulation of T cell activity influencing proliferation and cytokine production.

Biological function summary

ICOSL contributes to the modulation of immune responses. By forming a complex with the active ICOS protein ICOSL is involved in the co-stimulation of T cells. This process is important for the development of T-cell-dependent immune responses and helps in promoting the survival and differentiation of T cells. Together the ICOS ligand and ICOS protein create a dynamic interaction that balances immune activation and tolerance which is essential for maintaining immune homeostasis.

Pathways

The ICOS-ICOSL interaction plays an important role in the T-cell receptor (TCR) signaling pathway. This pathway is fundamental for the activation of T cells and subsequent immune response. ICOSL also connects to the PI3K/Akt pathway where it impacts cell survival and proliferation. ICOS and the PI3K/Akt pathway work together to influence various aspects of immune regulation and adaptive immunity.

ICOSL has a notable impact on autoimmune diseases and cancer. Its function in immune modulation when dysregulated can contribute to autoimmune conditions such as systemic lupus erythematosus. In this context the abnormal activation of the ICOS protein may exaggerate immune responses leading to pathology. In cancer high expression of ICOSL can support tumor progression by affecting the immune surveillance capability. These associations reveal ICOSL’s role in balancing immune activation and inhibition which is important in the context of disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed : 11007762, PubMed : 11023515, PubMed : 30498080). Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP) (PubMed : 30498080).

See full target information ICOSLG

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [ICOSL/3111] - BSA and Azide free (AB259280)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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