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AB66495

Anti-Id1 antibody [2456C1a]

4

(1 Review)

|

(5 Publications)

Mouse Monoclonal ID1 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ID1.

View Alternative Names

BHLHB24, ID, ID1, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, bHLHb24

1 Images
Western blot - Anti-Id1 antibody [2456C1a] (AB66495)
  • WB

Unknown

Western blot - Anti-Id1 antibody [2456C1a] (AB66495)

Western blot analysis of immunized recombinant protein, using ab66495.

All lanes:

Western blot - Anti-Id1 antibody [2456C1a] (ab66495)

Predicted band size: 16 kDa

Observed band size: 34 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2456C1a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ID1. The exact immunogen used to generate this antibody is proprietary information.

P41134

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (~95%) fetal bovine serum.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Id1 protein also known as Inhibitor of Differentiation 1 functions as a helix-loop-helix (HLH) transcription factor. It belongs to the family of inhibitor of differentiation proteins and weighs approximately 16 kDa. It lacks a basic DNA-binding domain which causes it to prevent transcription when it forms heterodimers with other HLH proteins. Id1 shows expression in various tissues but has noticeably high levels in embryonic and adult tissues involved in developmental processes.
Biological function summary

The Id1 protein plays significant roles in cellular differentiation proliferation and senescence. It does not function within a larger complex but acts as a suppressor of basic HLH transcription factors influencing cell differentiation pathways. Particularly it inhibits functions of factors that promote differentiation allowing cells to maintain a proliferative state. This inhibition capability marks Id1 as an important regulator in development and adult tissue maintenance.

Pathways

The Id1 protein influences key processes by interacting with the TGF-β (transforming growth factor-beta) pathway. It helps regulate cellular responses to growth factors that control cell cycle and apoptosis. In addition the protein has connections to the Notch signaling pathway which is important in cell fate determination. Through these pathways Id1 interacts with proteins such as Smad and Notch receptors highlighting its influence on developmental and immune responses.

Id1 has been linked to cancer and neurodegenerative diseases. Its dysregulation often leads to abnormal cell proliferation and resistance to differentiation processes that are hallmarks in cancer progression. Related proteins like c-Myc have interactions with Id1 in cancer endorsing cell cycle advancement. Additionally elevated Id1 expression connects to neurodegenerative conditions as it may disrupt normal neuronal differentiation linking to proteins such as NF-kB involved in cell survival and inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).
See full target information ID1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:1521 PubMed33750801

2021

A noncanonical AR addiction drives enzalutamide resistance in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yundong He,Ting Wei,Zhenqing Ye,Jacob J Orme,Dong Lin,Haoyue Sheng,Ladan Fazli,R Jeffrey Karnes,Rafael Jimenez,Liguo Wang,Liewei Wang,Martin E Gleave,Yuzhuo Wang,Lei Shi,Haojie Huang

Arthritis & rheumatology (Hoboken, N.J.) 71:1241-1251 PubMed30861322

2019

Citrullinated Inhibitor of DNA Binding 1 Is a Novel Autoantigen in Rheumatoid Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ray A Ohara,Gautam Edhayan,Stephanie M Rasmussen,Takeo Isozaki,Henriette A Remmer,Thomas M Lanigan,Phillip L Campbell,Andrew G Urquhart,Jeffrey N Lawton,Kevin C Chung,David A Fox,Jeffrey H Ruth

Oncology reports 41:1091-1100 PubMed30535485

2018

Overexpression of ID1 promotes tumor progression in penile squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiheng Hu,Mingfeng Chen,Yangle Li,Yin Wang,Sailan Wen,Fu Jun

Molecular medicine reports 16:8907-8915 PubMed29039489

2017

ID1 contributes to cell growth invasion and migration in salivary adenoid cystic carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Meng Hu,Ting Lin,Xiao-Yu Huang,Rui-Huan Gan,Yong Zhao,Yan Feng,Lin-Can Ding,Bo-Hua Su,Da-Li Zheng,You-Guang Lu

Pathology oncology research : POR 19:437-46 PubMed23397264

2013

Expression pattern of id proteins in medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew D Snyder,Ashley N Dulin-Smith,Ronald H Houston,Ashley N Durban,Bethany J Brisbin,Tyler D Oostra,Jordan T Marshall,Basil M Kahwash,Christopher R Pierson
View all publications
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Product promise

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