Mouse Monoclonal ID1 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ID1.
Images
![Western blot - Anti-Id1 antibody [2456C1a] (AB66495), expandable thumbnail](https://content.abcam.com/products/images/id1-antibody-2456c1a-ab66495--western-blot-img161614.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Id1 antibody [2456C1a] (AB66495)")
Publications
Filter by
- Nature communications 12:15212021A noncanonical AR addiction drives enzalutamide resistance in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYundong He,Ting Wei,Zhenqing Ye,Jacob J Orme,Dong Lin,Haoyue Sheng,Ladan Fazli,R Jeffrey Karnes,Rafael Jimenez,Liguo Wang,Liewei Wang,Martin E Gleave,Yuzhuo Wang,Lei Shi,Haojie HuangPubMed 33750801
- Arthritis & rheumatology (Hoboken, N.J.) 71:1241-12512019Citrullinated Inhibitor of DNA Binding 1 Is a Novel Autoantigen in Rheumatoid Arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRay A Ohara et. al.PubMed 30861322
- Molecular medicine reports 16:8907-89152017ID1 contributes to cell growth invasion and migration in salivary adenoid cystic carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Meng Hu,Ting Lin,Xiao-Yu Huang,Rui-Huan Gan,Yong Zhao,Yan Feng,Lin-Can Ding,Bo-Hua Su,Da-Li Zheng,You-Guang LuPubMed 29039489
- Pathology oncology research : POR 19:437-462013Expression pattern of id proteins in medulloblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew D Snyder et. al.PubMed 23397264
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human ID1. The exact immunogen used to generate this antibody is proprietary information. Database link P41134
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant fragment - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).
Alternative names
BHLHB24, ID, ID1, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, bHLHb24
Recommended products
Mouse Monoclonal ID1 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ID1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human ID1. The exact immunogen used to generate this antibody is proprietary information. Database link P41134
- Clone number
- 2456C1a
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (~95%) fetal bovine serum.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Id1 protein also known as Inhibitor of Differentiation 1 functions as a helix-loop-helix (HLH) transcription factor. It belongs to the family of inhibitor of differentiation proteins and weighs approximately 16 kDa. It lacks a basic DNA-binding domain which causes it to prevent transcription when it forms heterodimers with other HLH proteins. Id1 shows expression in various tissues but has noticeably high levels in embryonic and adult tissues involved in developmental processes.
- Biological function summary
The Id1 protein plays significant roles in cellular differentiation proliferation and senescence. It does not function within a larger complex but acts as a suppressor of basic HLH transcription factors influencing cell differentiation pathways. Particularly it inhibits functions of factors that promote differentiation allowing cells to maintain a proliferative state. This inhibition capability marks Id1 as an important regulator in development and adult tissue maintenance.
- Pathways
The Id1 protein influences key processes by interacting with the TGF-β (transforming growth factor-beta) pathway. It helps regulate cellular responses to growth factors that control cell cycle and apoptosis. In addition the protein has connections to the Notch signaling pathway which is important in cell fate determination. Through these pathways Id1 interacts with proteins such as Smad and Notch receptors highlighting its influence on developmental and immune responses.
- Associated diseases and disorders
Id1 has been linked to cancer and neurodegenerative diseases. Its dysregulation often leads to abnormal cell proliferation and resistance to differentiation processes that are hallmarks in cancer progression. Related proteins like c-Myc have interactions with Id1 in cancer endorsing cell cycle advancement. Additionally elevated Id1 expression connects to neurodegenerative conditions as it may disrupt normal neuronal differentiation linking to proteins such as NF-kB involved in cell survival and inflammation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Id1 antibody [2456C1a] (ab66495), expandable thumbnail](https://content.abcam.com/products/images/id1-antibody-2456c1a-ab66495--western-blot-img161614.jpg "Western blot - Anti-Id1 antibody [2456C1a] (ab66495)")
Western blot - Anti-Id1 antibody [2456C1a] (ab66495)
Western blot analysis of immunized recombinant protein, using ab66495.
All lanes: Western blot - Anti-Id1 antibody [2456C1a] (ab66495)
Predicted band size: 16 kDa
Observed band size: 34 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com