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AB168256

Anti-Id1 antibody

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(9 Publications)

Mouse Polyclonal ID1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ID1.

View Alternative Names

BHLHB24, ID, ID1, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, bHLHb24

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Id1 antibody (AB168256)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Id1 antibody (AB168256)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Id1 using ab168256 at 10 μg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining.

Western blot - Anti-Id1 antibody (AB168256)
  • WB

Unknown

Western blot - Anti-Id1 antibody (AB168256)

All lanes:

Western blot - Anti-Id1 antibody (ab168256) at 1/500 dilution

Lane 1:

ID1-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 16 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Native Full Length Protein corresponding to Human ID1.

P41134

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Id1 protein also known as Inhibitor of Differentiation 1 functions as a helix-loop-helix (HLH) transcription factor. It belongs to the family of inhibitor of differentiation proteins and weighs approximately 16 kDa. It lacks a basic DNA-binding domain which causes it to prevent transcription when it forms heterodimers with other HLH proteins. Id1 shows expression in various tissues but has noticeably high levels in embryonic and adult tissues involved in developmental processes.
Biological function summary

The Id1 protein plays significant roles in cellular differentiation proliferation and senescence. It does not function within a larger complex but acts as a suppressor of basic HLH transcription factors influencing cell differentiation pathways. Particularly it inhibits functions of factors that promote differentiation allowing cells to maintain a proliferative state. This inhibition capability marks Id1 as an important regulator in development and adult tissue maintenance.

Pathways

The Id1 protein influences key processes by interacting with the TGF-β (transforming growth factor-beta) pathway. It helps regulate cellular responses to growth factors that control cell cycle and apoptosis. In addition the protein has connections to the Notch signaling pathway which is important in cell fate determination. Through these pathways Id1 interacts with proteins such as Smad and Notch receptors highlighting its influence on developmental and immune responses.

Id1 has been linked to cancer and neurodegenerative diseases. Its dysregulation often leads to abnormal cell proliferation and resistance to differentiation processes that are hallmarks in cancer progression. Related proteins like c-Myc have interactions with Id1 in cancer endorsing cell cycle advancement. Additionally elevated Id1 expression connects to neurodegenerative conditions as it may disrupt normal neuronal differentiation linking to proteins such as NF-kB involved in cell survival and inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).
See full target information ID1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 25:316 PubMed39160536

2024

Levosimendan mediates the BMP/Smad axis through upregulation of circUSP34-targeted miR-1298 to alleviate pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Meng,Linhong Song,Hui Wang,Gang Wang,Gengxu Zhou

American journal of cancer research 13:4115-4124 PubMed37818083

2023

TBX3 promotes the epithelial mesenchymal transition of cervical cancer by upregulating ID1.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Yang,Yanan Sun,Xiaopeng Jia,Yuru Cai,Xingnan Zhao,Nan Li

Thorax 77:1176-1186 PubMed35580897

2022

Relationship between impaired BMP signalling and clinical risk factors at early-stage vascular injury in the preterm infant.

Applications

Unspecified application

Species

Unspecified reactive species

Motaharehsadat Heydarian,Prajakta Oak,Xin Zhang,Nona Kamgari,Alida Kindt,Markus Koschlig,Tina Pritzke,Erika Gonzalez-Rodriguez,Kai Förster,Rory E Morty,Friederike Häfner,Christoph Hübener,Andreas W Flemmer,Ali Oender Yildirim,Deepti Sudheendra,Xuefei Tian,Agnese Petrera,Holger Kirsten,Peter Ahnert,Nick Morrell,Tushar J Desai,Jennifer Sucre,Edda Spiekerkoetter,Anne Hilgendorff

Aging 12: PubMed33493134

2021

Diagnostic and prognostic values of AKR1C3 and AKR1D1 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Zhu,Ruo Feng,Xu Lu,Yuan Liao,Zhicheng Du,Wenlong Zhai,Kunlun Chen

Journal of cellular physiology 234:21307-21315 PubMed31066042

2019

Growth/differentiation 5 promotes the differentiation of retinal stem cells into neurons via Atoh8.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxiu Li,Haibo Li,LuSi Zhang,Siqi Xiong,ShiJin Wen,Xiaobo Xia,Xia Zhou

Molecular medicine reports 17:2853-2860 PubMed29207147

2017

Effect and mechanism of RNAi targeting WWTR1 on biological activity of gastric cancer cells SGC7901.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Fang-Liang Yang,Chun-Fu Zhu,Li-Ming Tang

Oncotarget 8:113002-113012 PubMed29348884

2017

Alpha-1-antitrypsin functions as a protective factor in preeclampsia through activating Smad2 and inhibitor of DNA binding 4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaling Feng,Nan Wang,Jianjuan Xu,Jinfang Zou,Xi Liang,Huan Liu,Ying Chen

Nature communications 8:1454 PubMed29129908

2017

TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Deguan Lv,Yanxin Li,Weiwei Zhang,Angel A Alvarez,Lina Song,Jianming Tang,Wei-Qiang Gao,Bo Hu,Shi-Yuan Cheng,Haizhong Feng

Oncology letters 13:3014-3024 PubMed28529558

2017

siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Li,Xiaoyong Wei,Baofeng Wu,Yuanli Xiao,Mingzhu Yin,Qiaohong Yang
View all publications
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