Mouse Polyclonal ID1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ID1.
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Publications
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- American journal of cancer research 13:4115-41242023TBX3 promotes the epithelial mesenchymal transition of cervical cancer by upregulating ID1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyu Yang,Yanan Sun,Xiaopeng Jia,Yuru Cai,Xingnan Zhao,Nan LiPubMed 37818083
- Thorax 77:1176-11862022Relationship between impaired BMP signalling and clinical risk factors at early-stage vascular injury in the preterm infant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMotaharehsadat Heydarian,Prajakta Oak,Xin Zhang,Nona Kamgari,Alida Kindt,Markus Koschlig,Tina Pritzke,Erika Gonzalez-Rodriguez,Kai Förster,Rory E Morty,Friederike Häfner,Christoph Hübener,Andreas W Flemmer,Ali Oender Yildirim,Deepti Sudheendra,Xuefei Tian,Agnese Petrera,Holger Kirsten,Peter Ahnert,Nick Morrell,Tushar J Desai,Jennifer Sucre,Edda Spiekerkoetter,Anne HilgendorffPubMed 35580897
- Aging 12:2021Diagnostic and prognostic values of AKR1C3 and AKR1D1 in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengfei Zhu et. al.PubMed 33493134
- Journal of cellular physiology 234:21307-213152019Growth/differentiation 5 promotes the differentiation of retinal stem cells into neurons via Atoh8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanxiu Li et. al.PubMed 31066042
- Molecular medicine reports 17:2853-28602017Effect and mechanism of RNAi targeting WWTR1 on biological activity of gastric cancer cells SGC7901.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Li,Fang-Liang Yang,Chun-Fu Zhu,Li-Ming TangPubMed 29207147
- Oncotarget 8:113002-1130122017Alpha-1-antitrypsin functions as a protective factor in preeclampsia through activating Smad2 and inhibitor of DNA binding 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaling Feng et. al.PubMed 29348884
- Nature communications 8:14542017TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeguan Lv et. al.PubMed 29129908
- Oncology letters 13:3014-30242017siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinlin Li et. al.PubMed 28529558
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Human ID1. Database link P41134
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Sheep | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes ab168256 must be purified for ICC/IF application. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).
Alternative names
BHLHB24, ID, ID1, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, bHLHb24
Recommended products
Mouse Polyclonal ID1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ID1.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Human ID1. Database link P41134
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Id1 protein also known as Inhibitor of Differentiation 1 functions as a helix-loop-helix (HLH) transcription factor. It belongs to the family of inhibitor of differentiation proteins and weighs approximately 16 kDa. It lacks a basic DNA-binding domain which causes it to prevent transcription when it forms heterodimers with other HLH proteins. Id1 shows expression in various tissues but has noticeably high levels in embryonic and adult tissues involved in developmental processes.
- Biological function summary
The Id1 protein plays significant roles in cellular differentiation proliferation and senescence. It does not function within a larger complex but acts as a suppressor of basic HLH transcription factors influencing cell differentiation pathways. Particularly it inhibits functions of factors that promote differentiation allowing cells to maintain a proliferative state. This inhibition capability marks Id1 as an important regulator in development and adult tissue maintenance.
- Pathways
The Id1 protein influences key processes by interacting with the TGF-β (transforming growth factor-beta) pathway. It helps regulate cellular responses to growth factors that control cell cycle and apoptosis. In addition the protein has connections to the Notch signaling pathway which is important in cell fate determination. Through these pathways Id1 interacts with proteins such as Smad and Notch receptors highlighting its influence on developmental and immune responses.
- Associated diseases and disorders
Id1 has been linked to cancer and neurodegenerative diseases. Its dysregulation often leads to abnormal cell proliferation and resistance to differentiation processes that are hallmarks in cancer progression. Related proteins like c-Myc have interactions with Id1 in cancer endorsing cell cycle advancement. Additionally elevated Id1 expression connects to neurodegenerative conditions as it may disrupt normal neuronal differentiation linking to proteins such as NF-kB involved in cell survival and inflammation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Id1 antibody (ab168256)
All lanes: Western blot - Anti-Id1 antibody (ab168256) at 1/500 dilution
Lane 1: ID1-transfected 293T cell lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
Predicted band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Id1 antibody (ab168256)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Id1 using ab168256 at 10 μg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining.
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