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AB203202

Anti-Id1 antibody

4

(3 Reviews)

|

(2 Publications)

Rabbit Polyclonal ID1 antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ID1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BHLHB24, ID, ID1, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, bHLHb24

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Id1 antibody (AB203202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Id1 antibody (AB203202)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human colon carcinoma tissue sections labeling Id1 with ab203202 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Id1 antibody (AB203202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Id1 antibody (AB203202)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat brain tissue sections labeling Id1 with ab203202 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Id1 antibody (AB203202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Id1 antibody (AB203202)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse lymphoma tissue sections labeling Id1 with ab203202 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ID1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P41134

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Id1 protein also known as Inhibitor of Differentiation 1 functions as a helix-loop-helix (HLH) transcription factor. It belongs to the family of inhibitor of differentiation proteins and weighs approximately 16 kDa. It lacks a basic DNA-binding domain which causes it to prevent transcription when it forms heterodimers with other HLH proteins. Id1 shows expression in various tissues but has noticeably high levels in embryonic and adult tissues involved in developmental processes.
Biological function summary

The Id1 protein plays significant roles in cellular differentiation proliferation and senescence. It does not function within a larger complex but acts as a suppressor of basic HLH transcription factors influencing cell differentiation pathways. Particularly it inhibits functions of factors that promote differentiation allowing cells to maintain a proliferative state. This inhibition capability marks Id1 as an important regulator in development and adult tissue maintenance.

Pathways

The Id1 protein influences key processes by interacting with the TGF-β (transforming growth factor-beta) pathway. It helps regulate cellular responses to growth factors that control cell cycle and apoptosis. In addition the protein has connections to the Notch signaling pathway which is important in cell fate determination. Through these pathways Id1 interacts with proteins such as Smad and Notch receptors highlighting its influence on developmental and immune responses.

Id1 has been linked to cancer and neurodegenerative diseases. Its dysregulation often leads to abnormal cell proliferation and resistance to differentiation processes that are hallmarks in cancer progression. Related proteins like c-Myc have interactions with Id1 in cancer endorsing cell cycle advancement. Additionally elevated Id1 expression connects to neurodegenerative conditions as it may disrupt normal neuronal differentiation linking to proteins such as NF-kB involved in cell survival and inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).
See full target information ID1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:1327 PubMed40826447

2025

Spatially resolved analysis of TGF/BMP signalling in pancreatic ductal adenocarcinoma by digital pathology identifies patient subgroups with adverse outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Konstantin Bräutigam,Philipp Zens,Stefan Reinhard,Jessica L Rohrbach,Simon J Leedham,Anna S Wenning,Beat Gloor,Viktor H Koelzer,Martin Wartenberg

Bone research 6:22 PubMed30038821

2018

Oxidized phospholipids are ligands for LRP6.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Yu Chai,Changjun Li,Haiyun Liu,Weiping Su,Xiaonan Liu,Bing Yu,Weiqi Lei,Bin Yu,Janet L Crane,Xu Cao,Mei Wan
View all publications
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Product promise

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