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AB230679

Anti-Id1 antibody

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(7 Publications)

Rabbit Polyclonal ID1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ID1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BHLHB24, ID, ID1, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, bHLHb24

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Id1 antibody (AB230679)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Id1 antibody (AB230679)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Id1 with ab230679 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Id1 antibody (AB230679)
  • WB

Supplier Data

Western blot - Anti-Id1 antibody (AB230679)

All lanes:

Western blot - Anti-Id1 antibody (ab230679) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

Mouse liver whole cell lysate

Lane 4:

Rat liver whole cell lysate

Predicted band size: 16 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ID1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P41134

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Reactivity data

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AB315051

Human ID1 ELISA Kit

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View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Id1 protein also known as Inhibitor of Differentiation 1 functions as a helix-loop-helix (HLH) transcription factor. It belongs to the family of inhibitor of differentiation proteins and weighs approximately 16 kDa. It lacks a basic DNA-binding domain which causes it to prevent transcription when it forms heterodimers with other HLH proteins. Id1 shows expression in various tissues but has noticeably high levels in embryonic and adult tissues involved in developmental processes.
Biological function summary

The Id1 protein plays significant roles in cellular differentiation proliferation and senescence. It does not function within a larger complex but acts as a suppressor of basic HLH transcription factors influencing cell differentiation pathways. Particularly it inhibits functions of factors that promote differentiation allowing cells to maintain a proliferative state. This inhibition capability marks Id1 as an important regulator in development and adult tissue maintenance.

Pathways

The Id1 protein influences key processes by interacting with the TGF-β (transforming growth factor-beta) pathway. It helps regulate cellular responses to growth factors that control cell cycle and apoptosis. In addition the protein has connections to the Notch signaling pathway which is important in cell fate determination. Through these pathways Id1 interacts with proteins such as Smad and Notch receptors highlighting its influence on developmental and immune responses.

Id1 has been linked to cancer and neurodegenerative diseases. Its dysregulation often leads to abnormal cell proliferation and resistance to differentiation processes that are hallmarks in cancer progression. Related proteins like c-Myc have interactions with Id1 in cancer endorsing cell cycle advancement. Additionally elevated Id1 expression connects to neurodegenerative conditions as it may disrupt normal neuronal differentiation linking to proteins such as NF-kB involved in cell survival and inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).
See full target information ID1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of chemical neuroanatomy 132:102318 PubMed37482144

2023

Cleavage of semaphorin 4 C interferes with the neuroprotective effect of the semaphorin 4 C/Plexin B2 pathway on experimental intracerebral hemorrhage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Li,Xiang Li,Jiahe Wang,Haiying Li,Haitao Shen,Xiang Xu,Gang Chen

International journal of oncology 62: PubMed36928315

2023

The ALK1‑Smad1/5‑ID1 pathway participates in tumour angiogenesis induced by low‑dose photodynamic therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiya Guo,Yajuan Niu,Wang Han,Xiaoyu Han,Qing Chen,Si Tian,Ying Zhu,Dingqun Bai,Kaiting Li

Scientific reports 12:13555 PubMed35941362

2022

ID1 marks the tumorigenesis of pancreatic ductal adenocarcinoma in mouse and human.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanxin Tang,Sheng Zhang,Jiazi Li,Chunli Wu,Qing Fan

International journal of molecular sciences 23: PubMed35163396

2022

BMP9-ID1 Signaling Activates HIF-1α and VEGFA Expression to Promote Tumor Angiogenesis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Han Chen,Kouki Nio,Hong Tang,Taro Yamashita,Hikari Okada,Yingyi Li,Phuong Thi Bich Doan,Ru Li,Junyan Lv,Yoshio Sakai,Tatsuya Yamashita,Eishiro Mizukoshi,Masao Honda,Shuichi Kaneko

Molecules (Basel, Switzerland) 26: PubMed34500601

2021

The Coffee Diterpene, Kahweol, Ameliorates Pancreatic β-Cell Function in Streptozotocin (STZ)-Treated Rat INS-1 Cells through NF-kB and p-AKT/Bcl-2 Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Waseem El-Huneidi,Shabana Anjum,Khuloud Bajbouj,Eman Abu-Gharbieh,Jalal Taneera

Frontiers in cell and developmental biology 9:608396 PubMed33644047

2021

BMPER Ameliorates Renal Fibrosis by Inhibiting Tubular Dedifferentiation and Fibroblast Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xie,Zunen Xia,Wei Wang,Xiangjun Zhou,Changgeng Xu

Journal of the American Heart Association 9:e016586 PubMed32750294

2020

Vital Roles of Gremlin-1 in Pulmonary Arterial Hypertension Induced by Systemic-to-Pulmonary Shunts.

Applications

Unspecified application

Species

Unspecified reactive species

Liukun Meng,Xiao Teng,Yao Liu,Chao Yang,Shengwei Wang,Wen Yuan,Jian Meng,Hongjie Chi,Lihua Duan,Xiaoyan Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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