AB327137

Anti-ID1 Antibody [CL11995]

Name: Anti-ID1 Antibody [CL11995] (ab327137) | Abcam
Brand: Abcam Limited
SKU: AB327137
Price: 580 USD
Availability: InStock

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Mouse Monoclonal ID1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ID1.

View Alternative Names

BHLHB24, ID, ID1, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, bHLHb24

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-ID1 Antibody [CL11995] (AB327137)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ID1 Antibody [CL11995] (AB327137)

Immunofluorescence staining of PC-3 cells using ab327137 at 2–10 µg/ml, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID1 Antibody [CL11995] (AB327137)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID1 Antibody [CL11995] (AB327137)

Immunohistochemical staining of human liver using ab327137 at 1 : 500–1 : 1000 dilution, showing very weak nuclear positivity in hepatocytes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID1 Antibody [CL11995] (AB327137)

Immunohistochemical staining of human endometrium using ab327137 at 1 : 500–1 : 1000 dilution, showing strong cytoplasmic and nuclear positivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID1 Antibody [CL11995] (AB327137)

Immunohistochemical staining of human colon using ab327137 at 1 : 500–1 : 1000 dilution, showing moderate to strong cytoplasmic and nuclear positivity in glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID1 Antibody [CL11995] (AB327137)

Immunohistochemical staining of human cerebellum using ab327137 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in cells in molecular layer.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID1 Antibody [CL11995] (AB327137)

Immunohistochemical staining of human seminal vesicle using ab327137 at 1 : 500–1 : 1000 dilution, showing moderate nuclear positivity in a subset of glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID1 Antibody [CL11995] (AB327137)

Immunohistochemical staining of human glioblastoma multiforme using ab327137 at 1 : 500–1 : 1000 dilution, showing strong positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID1 Antibody [CL11995] (AB327137)

Immunohistochemistry analysis in human seminal vesicle and liver tissues using ab327137 at 1 : 500–1 : 1000 dilution. Corresponding ID1 RNA-seq data are presented for the same tissues.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL11995

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human ID1.

P41134

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).

See full target information ID1

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Product promise

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