AB316793

Anti-ID1 antibody [EPR24304-94] - BSA and Azide free (Detector)

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal ID1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

BHLHB24, ID, ID1, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, bHLHb24

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Sandwich ELISA - Anti-ID1 antibody [EPR24304-94] - BSA and Azide free (Detector) (AB316793)

sELISA

Supplier Data

Sandwich ELISA - Anti-ID1 antibody [EPR24304-94] - BSA and Azide free (Detector) (AB316793)

Sandwich ELISA of ab316791 with the capture antibody ab316792 dilution at 2 ug/mL and detector antibody ab316793 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24304-94

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab316793 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ID1. The recommended pair for sandwich ELISA is:

Capture: ab316792, Human ID1 Capture Antibody (unconjugated)

Detector: ab316793, Human ID1 Detector Antibody (unconjugated)

The reference range value is 7.81-1000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Id1 protein also known as Inhibitor of Differentiation 1 functions as a helix-loop-helix (HLH) transcription factor. It belongs to the family of inhibitor of differentiation proteins and weighs approximately 16 kDa. It lacks a basic DNA-binding domain which causes it to prevent transcription when it forms heterodimers with other HLH proteins. Id1 shows expression in various tissues but has noticeably high levels in embryonic and adult tissues involved in developmental processes.

Biological function summary

The Id1 protein plays significant roles in cellular differentiation proliferation and senescence. It does not function within a larger complex but acts as a suppressor of basic HLH transcription factors influencing cell differentiation pathways. Particularly it inhibits functions of factors that promote differentiation allowing cells to maintain a proliferative state. This inhibition capability marks Id1 as an important regulator in development and adult tissue maintenance.

Pathways

The Id1 protein influences key processes by interacting with the TGF-β (transforming growth factor-beta) pathway. It helps regulate cellular responses to growth factors that control cell cycle and apoptosis. In addition the protein has connections to the Notch signaling pathway which is important in cell fate determination. Through these pathways Id1 interacts with proteins such as Smad and Notch receptors highlighting its influence on developmental and immune responses.

Id1 has been linked to cancer and neurodegenerative diseases. Its dysregulation often leads to abnormal cell proliferation and resistance to differentiation processes that are hallmarks in cancer progression. Related proteins like c-Myc have interactions with Id1 in cancer endorsing cell cycle advancement. Additionally elevated Id1 expression connects to neurodegenerative conditions as it may disrupt normal neuronal differentiation linking to proteins such as NF-kB involved in cell survival and inflammation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer (By similarity).

See full target information ID1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ID1 antibody [EPR24304-94] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-ID1 antibody [EPR24304-94] - BSA and Azide free (Detector) (AB316793)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise