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AB53545

Anti-ID2 antibody [2457C5a]

5

(1 Review)

|

(1 Publication)

Mouse Monoclonal ID2 antibody 2457C5a. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 1 publication.

View Alternative Names

BHLHB26, ID2, DNA-binding protein inhibitor ID-2, Class B basic helix-loop-helix protein 26, Inhibitor of DNA binding 2, Inhibitor of differentiation 2, bHLHb26

1 Images
Western blot - Anti-ID2 antibody [2457C5a] (AB53545)
  • WB

Unknown

Western blot - Anti-ID2 antibody [2457C5a] (AB53545)

All lanes:

Western blot - Anti-ID2 antibody [2457C5a] (ab53545)

All lanes:

immunised recombinant protein

Predicted band size: 14 kDa

Observed band size: 32 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2457C5a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ID2. The exact immunogen used to generate this antibody is proprietary information.

Q02363

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab53545 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ID2 also known as inhibitor of DNA binding 2 is a nuclear protein with a molecular weight approximately 15 kDa. It functions as a dominant negative regulator and lacks a DNA-binding domain but it forms heterodimers with basic helix-loop-helix (bHLH) transcription factors. This interaction with bHLH proteins inhibits their ability to bind DNA and initiate transcription. ID2 expresses in various tissues particularly in places with high cellular proliferation such as the nervous system and developing tissues.
Biological function summary

ID2 plays a role in cellular proliferation differentiation and development by modulating the activity of other transcription factors. It acts as part of a regulatory complex that blocks differentiation pathways allowing cells to maintain a proliferative and less differentiated state. ID2 often interacts with proteins like E2A and MyoD which are important for muscle cell differentiation. By regulating these factors ID2 influences the balance between cell growth and terminal differentiation.

Pathways

ID2 integrates into key signaling pathways particularly the TGF-beta and BMP signaling pathways. Through its interaction with the aforementioned bHLH transcription factors ID2 indirectly influences processes governed by these pathways. TGF-beta pathway for instance regulates cell growth and differentiation in a context-dependent manner incorporating ID2's regulatory effect on differentiation-suppressing transcription factors.

ID2 has implications in cancer and neurodegenerative diseases. Dysregulation of ID2 expression or function can contribute to tumorigenesis as it affects cell cycle progression and prevents differentiation both important in cancer development. Additionally ID2 has connections to proteins like pRb in retinoblastoma where it influences the pathway that leads to uncontrolled cellular proliferation. In the context of neurodegenerative disorders altered ID2 activity might impact neuronal differentiation providing links to cognitive deficits.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Restricts the CLOCK and BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock : in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.
See full target information ID2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:1280 PubMed30894532

2019

ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Cao,Jimena Trillo-Tinoco,Rosa A Sierra,Carmen Anadon,Wenjie Dai,Eslam Mohamed,Ling Cen,Tara L Costich,Anthony Magliocco,Douglas Marchion,Richard Klar,Sven Michel,Frank Jaschinski,Richard R Reich,Shikhar Mehrotra,Juan R Cubillos-Ruiz,David H Munn,Jose R Conejo-Garcia,Paulo C Rodriguez
View all publications
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Product promise

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