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AB166708

Anti-ID2 antibody [4E12G5]

2

(1 Review)

|

(10 Publications)

Mouse Monoclonal ID2 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ID2.

View Alternative Names

BHLHB26, ID2, DNA-binding protein inhibitor ID-2, Class B basic helix-loop-helix protein 26, Inhibitor of DNA binding 2, Inhibitor of differentiation 2, bHLHb26

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (AB166708)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (AB166708)

Immunohistochemical analysis of paraffin embedded, DAB-stained Human breast cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.

Flow Cytometry - Anti-ID2 antibody [4E12G5] (AB166708)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ID2 antibody [4E12G5] (AB166708)

Flow cytometric analysis of ID2-labeled SK N SH cells (green) and negative control cells (red), using ab166708 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (AB166708)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (AB166708)

Immunohistochemical analysis of paraffin embedded, DAB-stained Human rectum cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.

Western blot - Anti-ID2 antibody [4E12G5] (AB166708)
  • WB

Unknown

Western blot - Anti-ID2 antibody [4E12G5] (AB166708)

All lanes:

Western blot - Anti-ID2 antibody [4E12G5] (ab166708) at 1/500 dilution

All lanes:

recombinant Human ID2 protein at 1 µg

Predicted band size: 14 kDa

false

Flow Cytometry - Anti-ID2 antibody [4E12G5] (AB166708)
  • Flow Cyt

CiteAb

Flow Cytometry - Anti-ID2 antibody [4E12G5] (AB166708)

ID2 Flow cytometry/Cell sorting using Anti-ID2 antibody [4E12G5], ab166708. Publication image from Im, S. H. et al., 2018, Nat Commun, PubMed : 30413714.

Id2 is induced by IL-1β and IL-6 mediated signaling and downstream transcription factors STAT3, IRF4 and BATF in Treg cells. a Strategy for sorting TNaive, iTreg, and ex-Foxp3 TH17 cells from Foxp3Thy1.1 reporter mice (left). Flow cytometry analysis of IL-1r1 and IL-6rα among the TNaive, iTreg, and ex-Foxp3 TH17 cells (right). b, c FACS-sorted iTreg cells were re-stimulated with cytokines as indicated and analyzed for Id2 and Foxp3 mRNA expression by RT-qPCR (b) as well as for Id2 protein levels by flow cytometry (c). d Cartoon depicting highly putative STAT3, IRF4, and BATF binding motifs upstream of Id2 transcription start site (TSS). e Cartoon depicting Id2 promoter constructs used for luciferase reporter assay. f Id2 promoter-luciferase construct (−582/+36) were co-transfected with the combination of STAT3, IRF4 and BATF expressing vectors in HEK-293 T cells. Lysates were prepared 30 h after transfection, and luciferase activities were measured with the reporter activities normalized to renilla luciferase activity. *P 0.05, **P < 0.005, ***P < 0.001 (Student’s t-test). All data are representative three independent experiments with similar results (error bars, s.d.)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4E12G5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ID2.

Q02363

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ID2 also known as inhibitor of DNA binding 2 is a nuclear protein with a molecular weight approximately 15 kDa. It functions as a dominant negative regulator and lacks a DNA-binding domain but it forms heterodimers with basic helix-loop-helix (bHLH) transcription factors. This interaction with bHLH proteins inhibits their ability to bind DNA and initiate transcription. ID2 expresses in various tissues particularly in places with high cellular proliferation such as the nervous system and developing tissues.
Biological function summary

ID2 plays a role in cellular proliferation differentiation and development by modulating the activity of other transcription factors. It acts as part of a regulatory complex that blocks differentiation pathways allowing cells to maintain a proliferative and less differentiated state. ID2 often interacts with proteins like E2A and MyoD which are important for muscle cell differentiation. By regulating these factors ID2 influences the balance between cell growth and terminal differentiation.

Pathways

ID2 integrates into key signaling pathways particularly the TGF-beta and BMP signaling pathways. Through its interaction with the aforementioned bHLH transcription factors ID2 indirectly influences processes governed by these pathways. TGF-beta pathway for instance regulates cell growth and differentiation in a context-dependent manner incorporating ID2's regulatory effect on differentiation-suppressing transcription factors.

ID2 has implications in cancer and neurodegenerative diseases. Dysregulation of ID2 expression or function can contribute to tumorigenesis as it affects cell cycle progression and prevents differentiation both important in cancer development. Additionally ID2 has connections to proteins like pRb in retinoblastoma where it influences the pathway that leads to uncontrolled cellular proliferation. In the context of neurodegenerative disorders altered ID2 activity might impact neuronal differentiation providing links to cognitive deficits.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Restricts the CLOCK and BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock : in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.
See full target information ID2
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 19:3324-3340 PubMed37497010

2023

SMURF2 facilitates ubiquitin-mediated degradation of ID2 to attenuate lung cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Mingwei Han,Yixiao Guo,Yiming Li,Qingmei Zeng,Wanwan Zhu,Jianli Jiang

Frontiers in oncology 12:801300 PubMed35982951

2022

Id2 exerts tumor suppressor properties in lung cancer through its effects on cancer cell invasion and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Ting Chen,Yuan-Ling Hsu,Yi-Chiung Hsu,Yi-Hsin Tseng,Ming-Han Liu,Chia-Wei Weng,Ching-Hao Lin,Szu-Hua Pan,Jeremy J W Chen,Chi-Chung Wang

Physiological reports 9:e15119 PubMed34806343

2021

Unresolved intramuscular inflammation, not diminished skeletal muscle regenerative capacity, is at the root of rheumatoid cachexia: insights from a rat CIA model.

Applications

Unspecified application

Species

Unspecified reactive species

Tracey Ollewagen,Yigael S L Powrie,Kathryn H Myburgh,Carine Smith

Medical science monitor : international medical jo 25:4438-4449 PubMed31201297

2019

Phospholipase C (PLC)ε Promotes Androgen Receptor Antagonist Resistance via the Bone Morphogenetic Protein (BMP)-6/SMAD Axis in a Castration-Resistant Prostate Cancer Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjuan Yuan,Yingying Gao,Luo Li,Wei Sun,Honglin Cheng,Ting Li,Lingfang Niu,Zhongbo Du,Zhen Quan,Chunli Luo,Xiaohou Wu

Life sciences 227:64-73 PubMed31004656

2019

miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Liu,Xiao-Zhou Zou,Ning Huang,Xiao-Yue Ge,Mao-Zhong Yao,Hong Liu,Zheng Zhang,Chang-Ping Hu

Nature communications 9:4736 PubMed30413714

2018

Inflammation-induced Id2 promotes plasticity in regulatory T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Min Hwang,Garima Sharma,Ravi Verma,Seohyun Byun,Dipayan Rudra,Sin-Hyeog Im

eLife 7: PubMed30095408

2018

E proteins sharpen neurogenesis by modulating proneural bHLH transcription factors' activity in an E-box-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Gwenvael Le Dréau,René Escalona,Raquel Fueyo,Antonio Herrera,Juan D Martínez,Susana Usieto,Anghara Menendez,Sebastian Pons,Marian A Martinez-Balbas,Elisa Marti

Journal of cellular biochemistry 119:9284-9294 PubMed30010211

2018

Effect of Notch1 gene on remyelination in multiple sclerosis in mouse models of acute demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Fan,Jie-Gang Zhao,Jun-Qiang Yan,Gan-Qin Du,Qi-Zhi Fu,Jian Shi,Yan-Hui Yang,Xiao-Wei Du,Xiao-Li Bai

Oncotarget 8:113002-113012 PubMed29348884

2017

Alpha-1-antitrypsin functions as a protective factor in preeclampsia through activating Smad2 and inhibitor of DNA binding 4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaling Feng,Nan Wang,Jianjuan Xu,Jinfang Zou,Xi Liang,Huan Liu,Ying Chen

Cell 171:201-216.e18 PubMed28844693

2017

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Wang,Pengyan Xia,Yi Chen,Yuan Qu,Zhen Xiong,Buqing Ye,Ying Du,Yong Tian,Zhinan Yin,Zhiheng Xu,Zusen Fan
View all publications
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