Skip to main content

Rabbit Recombinant Monoclonal ID2 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human, Mouse samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (AB320647), expandable thumbnail
  • Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (AB320647), expandable thumbnail
  • Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (AB320647), expandable thumbnail
  • Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (AB320647), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIPICC/IF
Human
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human, Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Restricts the CLOCK and BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.

Alternative names

BHLHB26, ID2, DNA-binding protein inhibitor ID-2, Class B basic helix-loop-helix protein 26, Inhibitor of DNA binding 2, Inhibitor of differentiation 2, bHLHb26

Recommended products

Rabbit Recombinant Monoclonal ID2 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR29025-9
Purification technique
Affinity purification Protein A
Specificity

This antibody does not cross-react with overexpressed human ID1 (#P41134) by Western Blot.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab320647 is the carrier-free version of Anti-ID2 antibody [EPR29025-9] ab320646.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ID2 also known as inhibitor of DNA binding 2 is a nuclear protein with a molecular weight approximately 15 kDa. It functions as a dominant negative regulator and lacks a DNA-binding domain but it forms heterodimers with basic helix-loop-helix (bHLH) transcription factors. This interaction with bHLH proteins inhibits their ability to bind DNA and initiate transcription. ID2 expresses in various tissues particularly in places with high cellular proliferation such as the nervous system and developing tissues.

Biological function summary

ID2 plays a role in cellular proliferation differentiation and development by modulating the activity of other transcription factors. It acts as part of a regulatory complex that blocks differentiation pathways allowing cells to maintain a proliferative and less differentiated state. ID2 often interacts with proteins like E2A and MyoD which are important for muscle cell differentiation. By regulating these factors ID2 influences the balance between cell growth and terminal differentiation.

Pathways

ID2 integrates into key signaling pathways particularly the TGF-beta and BMP signaling pathways. Through its interaction with the aforementioned bHLH transcription factors ID2 indirectly influences processes governed by these pathways. TGF-beta pathway for instance regulates cell growth and differentiation in a context-dependent manner incorporating ID2's regulatory effect on differentiation-suppressing transcription factors.

Associated diseases and disorders

ID2 has implications in cancer and neurodegenerative diseases. Dysregulation of ID2 expression or function can contribute to tumorigenesis as it affects cell cycle progression and prevents differentiation both important in cancer development. Additionally ID2 has connections to proteins like pRb in retinoblastoma where it influences the pathway that leads to uncontrolled cellular proliferation. In the context of neurodegenerative disorders altered ID2 activity might impact neuronal differentiation providing links to cognitive deficits.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (ab320647), expandable thumbnail

    Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (ab320647)

    This data was developed using Anti-ID2 antibody [EPR29025-9] ab320646, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands higher than 20 kDa and lower than 14 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ID2 antibody [EPR29025-9] (Anti-ID2 antibody [EPR29025-9] ab320646) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 30 µg

    Lane 2: NIH/3T3 treated with 20μМM MG-132 for 4 hours whole cell lysate at 30 µg

    Lane 3: Untreated MEF (mouse embryo fibroblast) whole cell lysate at 30 µg

    Lane 4: MEF treated with 20μМM MG-132 for 4 hours whole cell lysate at 30 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 15 kDa, 36 kDa

    Exposure time: 48s

  • Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (ab320647), expandable thumbnail

    Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (ab320647)

    This data was developed using Anti-ID2 antibody [EPR29025-9] ab320646, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: A-204, HT-1080, RD (PMID: 12447693).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ID2 antibody [EPR29025-9] (Anti-ID2 antibody [EPR29025-9] ab320646) at 1/1000 dilution

    Lane 1: Untreated A-673 (human muscle ewings sarcoma cell line) whole cell lysate at 30 µg

    Lane 2: A-673 treated with 20μM MG-132 for 4 hours whole cell lysate at 30 µg

    Lane 3: Untreated HCT-15 (human colon epithelial cell) whole cell lysate at 30 µg

    Lane 4: HCT-15 treated with 20μM MG-132 for 4 hours whole cell lysate at 30 µg

    Lane 5: A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate at 30 µg

    Lane 6: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 30 µg

    Lane 7: RD (human muscle mtinucleated spindle-shaped cell) whole cell lysate at 30 µg

    Lane 8: Untreated HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 30 µg

    Lane 9: HCT 116 treated with 10μM MG-132 for 4 hours whole cell lysate at 30 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 15 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (ab320647), expandable thumbnail

    Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (ab320647)

    This data was developed using Anti-ID2 antibody [EPR29025-9] ab320646, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with overexpressed human ID1 by Western Blot.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-ID2 antibody [EPR29025-9] (Anti-ID2 antibody [EPR29025-9] ab320646) at 1/1000 dilution

    Lane 1: 293T cells transfected with a human ID2 expression vector containing a myc-His-tag® treated with 20μM MG-132 for 5 hours whole cell lysate at 10 µg

    Lane 2: 293T cells transfected with a human ID1 expression vector containing a myc-His-tag® treated with 20μM MG-132 for 5 hours whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 18 kDa, 36 kDa

    Exposure time: 3s

  • Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (ab320647), expandable thumbnail

    Western blot - Anti-ID2 antibody [EPR29025-9] - BSA and Azide free (ab320647)

    This data was developed using Anti-ID2 antibody [EPR29025-9] ab320646, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ID2 antibody [EPR29025-9] (Anti-ID2 antibody [EPR29025-9] ab320646) at 1/1000 dilution

    Lane 1: Untreated SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 30 µg

    Lane 2: SK-N-BE(2) treated with 20uM MG-132 for 4 hours whole cell lysate at 30 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 15 kDa, 36 kDa

    Exposure time: 180s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com