AB236505

Anti-ID3 antibody [OTI8B3]

Name: Anti-ID3 antibody [OTI8B3] (ab236505) | Abcam
Brand: Abcam Limited
SKU: AB236505

(1 Review)

(4 Publications)

Mouse Monoclonal ID3 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ID3.

View Alternative Names

1R21, BHLHB25, HEIR1, ID3, DNA-binding protein inhibitor ID-3, Class B basic helix-loop-helix protein 25, Helix-loop-helix protein HEIR-1, ID-like protein inhibitor HLH 1R21, Inhibitor of DNA binding 3, Inhibitor of differentiation 3, bHLHb25

10 Images

IHC-P

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Paraffin-embedded human kidney tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-ID3 antibody [OTI8B3] (AB236505)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ID3 antibody [OTI8B3] (AB236505)

Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ID3 with ab236505 at 1/100 dilution (red) compared to a negative control antibody (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Paraffin-embedded human prostate tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Paraffin-embedded human thyroid tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ID3 antibody [OTI8B3] (AB236505)

Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling ID3 with ab236505 at 1/100 dilution (red) compared to a negative control antibody (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Paraffin-embedded human ovary tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Paraffin-embedded human colon tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Paraffin-embedded human liver tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ID3 antibody [OTI8B3] (AB236505)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ID3 antibody [OTI8B3] (AB236505)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ID3 cDNA stained for ID3 (green) using ab236505 at 1/100 dilution in ICC/IF.

Supplier Data

Western blot - Anti-ID3 antibody [OTI8B3] (AB236505)

All lanes:

Western blot - Anti-ID3 antibody [OTI8B3] (ab236505) at 1/500 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cDNA, whole cell lysate at 5 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ID3 cDNA, whole cell lysate at 5 µg

Predicted band size: 13 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI8B3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ID3.

Q02535

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Clone OTI8B3 (formerly 8B3).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ID3 also known as Inhibitor of DNA Binding 3 is a helix-loop-helix (HLH) protein involved in the regulation of transcription. It weighs approximately 15 kDa. This protein is expressed in various tissues including the central nervous system hematopoietic cells and developing organs. ID3 antagonizes basic HLH transcription factors which prevents them from binding to DNA influencing gene expression and cellular differentiation processes important for development.

Biological function summary

ID3 plays a significant role in cellular growth and differentiation. It functions as part of a regulatory complex that inhibits the binding of other HLH proteins to E-box sequences on DNA modulating gene expression patterns. This modulation is vital during embryogenesis and adult tissue homeostasis by controlling the balance between cell proliferation and differentiation. The influence of ID3 extends to its involvement in neural development and immune cell maturation.

Pathways

ID3 integrates into the BMP signaling pathway and is closely connected with the Notch signaling pathway. Within these pathways it acts by influencing cellular decisions of differentiation and proliferation. ID3 interacts with E-proteins such as E12 and E47 negatively regulating their transcriptional activity. This regulation affects cell fate outcomes emphasizing ID3's versatile role in diverse biological settings.

ID3 has connections with lymphatic malignancies and neurodegenerative diseases. Overexpression of ID3 often associates with aggressive forms of lymphoma where it disrupts normal differentiation programs. In the nervous system dysregulation of ID3 may contribute to neurodevelopmental disorders. Proteins like MYC a known oncogene link with ID3 in cancer pathways highlighting ID3's importance in tumorigenesis and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.

See full target information ID3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 15:1247-1257 PubMed37065597

2023

-mediated m6A mRNA modification of suppresses cisplatin resistance in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xifan Wang,Yu'an Hu,Xiaoqing Li,Chuandong Zhu,Fangfang Chen

Disease markers 2023:8143581 PubMed36815135

2023

Transcription Activation of Rab8A by PEA3 Augments Progression of Esophagus Cancer by Activating the Wnt/-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shusheng Cai,Xiumei Liu,Weiran Gao,Lianhua Ye,Qiming Zhong,Xin Zhang

Bioengineered 12:12294-12307 PubMed34927533

2021

Heterogeneity affects the differentiation potential of dental follicle stem cells through the TGF-β signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhaosong,Fu Na,Guo Shuling,Liu Jiacheng,Wei Ran

Communications biology 4:563 PubMed33980982

2021

A follicular regulatory Innate Lymphoid Cell population impairs interactions between germinal center Tfh and B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Margaret H O'Connor,Roshell Muir,Marita Chakhtoura,Michael Fang,Eirini Moysi,Susan Moir,Alison J Carey,Alyssa Terk,Carmen N Nichols,Talibah Metcalf,Constantinos Petrovas,Mark J Cameron,Virginie Tardif,Elias K Haddad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ID3 antibody [OTI8B3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Flow Cytometry (Intracellular) - Anti-ID3 antibody [OTI8B3] (AB236505)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Flow Cytometry (Intracellular) - Anti-ID3 antibody [OTI8B3] (AB236505)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID3 antibody [OTI8B3] (AB236505)

Immunocytochemistry/ Immunofluorescence - Anti-ID3 antibody [OTI8B3] (AB236505)

Western blot - Anti-ID3 antibody [OTI8B3] (AB236505)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise