Rabbit Recombinant Monoclonal ID4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (AB273062), expandable thumbnail](https://content.abcam.com/products/images/id4-antibody-epr22323-36-bsa-and-azide-free-ab273062--western-blot-img7912.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (AB273062)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation (By similarity).
Alternative names
BHLHB27, ID4, DNA-binding protein inhibitor ID-4, Class B basic helix-loop-helix protein 27, Inhibitor of DNA binding 4, Inhibitor of differentiation 4, bHLHb27
Recommended products
Rabbit Recombinant Monoclonal ID4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22323-36
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab273062 is the carrier-free version of Anti-ID4 antibody [EPR22323-36] ab220166.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ID4 also known as Inhibitor of DNA Binding 4 is a member of the ID protein family that inhibits basic helix-loop-helix (bHLH) transcription factors. The protein ID4 has a mass of approximately 16 kDa. It lacks a DNA binding domain but can still regulate transcription by forming heterodimers with other bHLH proteins preventing their interaction with DNA. ID4 is expressed in various tissues with notable expression in the central nervous system mammary gland and testis.
- Biological function summary
ID4 plays a significant role in regulating cell proliferation differentiation and apoptosis. It acts within the context of a protein complex by inhibiting the activity of bHLH transcription factors involved in these processes. It maintains stem cell properties by preventing premature differentiation especially in neural progenitor cells. ID4 also participates in the development of several tissues including regulating the differentiation of myoblasts during muscle development.
- Pathways
ID4 is integral to the cell cycle and development pathways. It interacts with the p53 pathway by modulating transcription factors involved in cell cycle control and apoptosis. Additionally ID4 has connections with the Notch signaling pathway which is important for cell fate determination and differentiation. In these pathways ID4 often associates with HES and HEY proteins which are downstream transcriptional regulators influenced by Notch signaling.
- Associated diseases and disorders
Aberrant expression of ID4 is linked to several cancers including glioblastoma and breast cancer. In these diseases ID4 can either promote or inhibit tumor growth depending on the context and its interaction with other proteins like p21 which is a cyclin-dependent kinase inhibitor involved in cell cycle regulation. Furthermore changes in ID4 expression are implicated in neurodegenerative disorders due to its regulation of neural stem cell differentiation and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (ab273062), expandable thumbnail](https://content.abcam.com/products/images/id4-antibody-epr22323-36-bsa-and-azide-free-ab273062--western-blot-img7912.jpg "Western blot - Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (ab273062)")
Western blot - Anti-ID4 antibody [EPR22323-36] - BSA and Azide free (ab273062)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver (PMID:8139914).
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (Anti-ID4 antibody [EPR22323-36] ab220166).
All lanes: Western blot - Anti-ID4 antibody [EPR22323-36] (Anti-ID4 antibody [EPR22323-36] ab220166) at 1/5000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human ovary tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
Lane 4: Mouse testis tissue lysate at 20 µg
Lane 5: Mouse ovary tissue lysate at 20 µg
Lane 6: Mouse liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Downloads
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