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AB137568

Anti-IDH1 antibody

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(1 Publication)

Rabbit Polyclonal IDH1 antibody. Suitable for IHC-P, WB, IP and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IDH1 aa 1-350.

View Alternative Names

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody (AB137568)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody (AB137568)

Immunohistochemical analysis of paraffin embedded human colon carcinoma, labelling IDH1 with ab137568 at 1/100 dilution.

Immunoprecipitation - Anti-IDH1 antibody (AB137568)
  • IP

Supplier Data

Immunoprecipitation - Anti-IDH1 antibody (AB137568)

Samples were separated by 10% SDS-PAGE. The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-IDH1 antibody (ab137568) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate extract (Input) at 30 µg

Lane 2:

HepG2 whole cell lysate extract with rabbit IgG C

Lane 3:

HepG2 whole cell lysate extract (+)

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 47 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody (AB137568)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody (AB137568)

Immunohistochemical analysis of paraffin embedded rat duodenum tissue labelling IDH1 with ab137568 at 1/1000 dilution. Antigen retrieval was performed with citrate buffer, pH 6.0, 15 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody (AB137568)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody (AB137568)

Immunohistochemical analysis of paraffin embedded mouse brain tissue labelling IDH1 with ab137568 at 1/1000 dilution. Antigen retrieval was performed with citrate buffer, pH 6.0, 15 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody (AB137568)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody (AB137568)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue stained for IDH1 protein at cytoplasm with ab137568 at a 1/1000 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-IDH1 antibody (AB137568)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody (AB137568)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-IDH1 antibody (ab137568) at 1/1000 dilution

Lane 1:

Huh-7 whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 47 kDa

true

Western blot - Anti-IDH1 antibody (AB137568)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody (AB137568)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-IDH1 antibody (ab137568) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-taged IDH1 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 47 kDa

false

Western blot - Anti-IDH1 antibody (AB137568)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody (AB137568)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-IDH1 antibody (ab137568) at 1/5000 dilution

Lane 1:

Non-transfected (–) HepG2 whole cell extracts at 30 µg

Lane 2:

IDH1 shRNA transfected (+) HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 47 kDa

false

Western blot - Anti-IDH1 antibody (AB137568)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody (AB137568)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-IDH1 antibody (ab137568) at 1/1000 dilution

Lane 1:

Rat liver tissue extracts at 50 µg

Lane 2:

Rat heart tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 47 kDa

false

Western blot - Anti-IDH1 antibody (AB137568)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody (AB137568)

All lanes:

Western blot - Anti-IDH1 antibody (ab137568) at 1/500 dilution

Lane 1:

Wild-type HeLa whole cell lysate at 30 µg

Lane 2:

IDH1 knockout HeLa whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IDH1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

O75874

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/1000", "IP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Keep as concentrated solution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.
Biological function summary

Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.

Pathways

The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.

IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
See full target information IDH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 322:C624-C644 PubMed35196163

2022

PDIA3/ERp57 promotes a matrix-rich secretome that stimulates fibroblast adhesion through CCN2.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew L Hellewell,Kate J Heesom,Mark A Jepson,Josephine C Adams
View all publications
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