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AB239606

Anti-IDH1 antibody [C12]

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(2 Publications)

Mouse Monoclonal IDH1 antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IDH1 aa 50-350.

View Alternative Names

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)

Formalin-fixed, paraffin-embedded human glioma tissue stained for IDH1 with ab239606 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)

Formalin-fixed, paraffin-embedded human kidney tissue stained for IDH1 with ab239606 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)

Formalin-fixed, paraffin-embedded human prostate tissue stained for IDH1 with ab239606 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [C12] (AB239606)

Formalin-fixed, paraffin-embedded human stomach tissue stained for IDH1 with ab239606 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-IDH1 antibody [C12] (AB239606)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody [C12] (AB239606)

All lanes:

Western blot - Anti-IDH1 antibody [C12] (ab239606) at 5 µg/mL

All lanes:

Recombinant human IDH1 protein

Predicted band size: 46 kDa

false

Western blot - Anti-IDH1 antibody [C12] (AB239606)
  • WB

Supplier Data

Western blot - Anti-IDH1 antibody [C12] (AB239606)

All lanes:

Western blot - Anti-IDH1 antibody [C12] (ab239606) at 3 µg/mL

Lane 1:

Human liver tissue lysate

Lane 2:

Pig liver tissue lysate

Secondary

All lanes:

HRP-Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C12

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IDH1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

O75874

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.
Biological function summary

Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.

Pathways

The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.

IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
See full target information IDH1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eabp8293 PubMed36525494

2022

In vivo characterization of glutamine metabolism identifies therapeutic targets in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Akash K Kaushik,Amy Tarangelo,Lindsey K Boroughs,Mukundan Ragavan,Yuanyuan Zhang,Cheng-Yang Wu,Xiangyi Li,Kristen Ahumada,Jui-Chung Chiang,Vanina T Tcheuyap,Faeze Saatchi,Quyen N Do,Cissy Yong,Tracy Rosales,Christina Stevens,Aparna D Rao,Brandon Faubert,Panayotis Pachnis,Lauren G Zacharias,Hieu Vu,Feng Cai,Thomas P Mathews,Giannicola Genovese,Barbara S Slusher,Payal Kapur,Xiankai Sun,Matthew Merritt,James Brugarolas,Ralph J DeBerardinis

Journal of immunology (Baltimore, Md. : 1950) 158:1108-15 PubMed9013949

1997

Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells.

Applications

Unspecified application

Species

Unspecified reactive species

A M Oliver,F Martin,J F Kearney
View all publications
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