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AB172964

Anti-IDH1 antibody [EPR12296]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

5

(2 Reviews)

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(24 Publications)

Anti-IDH1 antibody [EPR12296] (ab172964) is a rabbit monoclonal antibody detecting IDH1 in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (AB172964)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (AB172964)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling IDH1 with purified ab172964 at 1/200 dilution (0.60 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (AB172964)
  • IP

Lab

Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (AB172964)

Purified ab172964 at 1/20 dilution (0.6μg) immunoprecipitating IDH1 in HepG2 whole cell lysate.
Lane 1 (input) : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate.
Lane 2 (+) : ab172964 + HepG2 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab172964 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (ab172964)

Predicted band size: 46 kDa

Observed band size: 47 kDa

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Western blot - Anti-IDH1 antibody [EPR12296] (AB172964)
  • WB

Lab

Western blot - Anti-IDH1 antibody [EPR12296] (AB172964)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : IDH1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HepG2 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab172964 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab172964 was shown to specifically react with IDH1 in wild-type HAP1 cells. No band was observed when IDH1 knockout samples were used. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE. ab172964 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)

Predicted band size: 46 kDa

false

Western blot - Anti-IDH1 antibody [EPR12296] (AB172964)
  • WB

Unknown

Western blot - Anti-IDH1 antibody [EPR12296] (AB172964)

All lanes:

Western blot - Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution

All lanes:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 46 kDa

false

Western blot - Anti-IDH1 antibody [EPR12296] (AB172964)
  • WB

Unknown

Western blot - Anti-IDH1 antibody [EPR12296] (AB172964)

All lanes:

Western blot - Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

Lane 2:

Rat kidney lysate

Lane 3:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12296

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-IDH1 antibody [EPR12296] (ab172964) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of IDH1?
Anti-IDH1 [EPR12296] (ab172964) specifically detects a band for IDH1 (UniProt: O75874) at a molecular weight of 47kDa.

Trusted by the scientific community
Anti-IDH1 [EPR12296] (ab172964) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-IDH1 antibody [EPR12296] (ab172964) has been confirmed by Western blot testing in IDH1 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR12296] also available for your convenience: ab172964, Carrier free - ab214803, Alexa Fluor® 488 - ab311026, Alexa Fluor® 647 - ab311152, Alexa Fluor® 594 - ab311797, Alexa Fluor® 568 - ab313080, Alexa Fluor® 555 - ab313274, Alexa Fluor® 750 - ab321175

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Shipping conditions update: ambient shipping

This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.

Why the change?

It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.

What you need to know

  • Ambient shipments come clearly marked on the delivery note.
  • No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
  • Warranty coverage remains fully valid, aligned with our validated shipping method.
  • Please store the product as per the datasheet instructions upon receipt.

Find out more - https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.
Biological function summary

Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.

Pathways

The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.

IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
See full target information IDH1
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 8:274 PubMed39587279

2024

Deep mutual learning on hybrid amino acid PET predicts H3K27M mutations in midline gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Yuan,Guanglei Li,Shuhao Mei,Mingtao Hu,Ying-Hua Chu,Yi-Cheng Hsu,Chaolin Li,Jianping Song,Jie Hu,Danyang Feng,Fang Xie,Yihui Guan,Qi Yue,Mianxin Liu,Ying Mao

STAR protocols 5:103200 PubMed39028619

2024

Protocol for transducing human primary epithelial prostate cells and patient-derived organoids with high efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia Jobin,Mario Harvey,Aurélie Lacouture,Cindy Weidmann,Bertrand Neveu,Frédéric Pouliot,Étienne Audet-Walsh

The EMBO journal 43:1113-1134 PubMed38418556

2024

Metabolic rewiring of macrophages by epidermal-derived lactate promotes sterile inflammation in the murine skin.

Applications

Unspecified application

Species

Unspecified reactive species

Uttkarsh Ayyangar,Aneesh Karkhanis,Heather Tay,Aliya Farissa Binte Afandi,Oindrila Bhattacharjee,Lalitha Ks,Sze Han Lee,James Chan,Srikala Raghavan

iScience 26:107206 PubMed37456829

2023

IDH1 K224 acetylation promotes colorectal cancer via miR-9-5p/NHE1 axis-mediated regulation of acidic microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wang,Long Zhao,Changjiang Yang,Yilin Lin,Shan Wang,Yingjiang Ye,Jianyuan Luo,Zhanlong Shen

Frontiers in oncology 13:1134626 PubMed37223677

2023

Convolutional neural network to predict IDH mutation status in glioma from chemical exchange saturation transfer imaging at 7 Tesla.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Yuan,Yang Yu,Jun Chang,Ying-Hua Chu,Wenwen Yu,Yi-Cheng Hsu,Liebig Alexander Patrick,Mianxin Liu,Qi Yue

Molecular oncology 17:2109-2125 PubMed37086156

2023

Isocitrate dehydrogenase 1 sustains a hybrid cytoplasmic-mitochondrial tricarboxylic acid cycle that can be targeted for therapeutic purposes in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Gonthier,Cindy Weidmann,Line Berthiaume,Cynthia Jobin,Aurélie Lacouture,Camille Lafront,Mario Harvey,Bertrand Neveu,Jérémy Loehr,Alain Bergeron,Yves Fradet,Louis Lacombe,Julie Riopel,Éva Latulippe,Chantal Atallah,Michael Shum,Jean-Philippe Lambert,Frédéric Pouliot,Martin Pelletier,Étienne Audet-Walsh

PloS one 18:e0283616 PubMed36996057

2023

Prognostic value of epigenetic markers for canine mast cell cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shahzar Syed,Britta J Knight,Geoffrey A Wood,Brenda Lynn Coomber

Journal of cellular and molecular medicine 27:259-265 PubMed36601689

2023

SIX4 upregulates IDH1 and metabolic reprogramming to promote osteosarcoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Li,Xiaoqian Dang,Jiafeng Duan,Guangyang Zhang,Jia Zhang,Qichun Song

PeerJ 10:e14052 PubMed36193432

2022

BRCA1 overexpression attenuates breast cancer cell growth and migration by regulating the pyruvate kinase M2-mediated Warburg effect the PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuli Liu,Hanxu Liu,Lei Zeng,Yuetao Lv

Cancer science 113:3032-3043 PubMed35730256

2022

Silybin suppresses ovarian cancer cell proliferation by inhibiting isocitrate dehydrogenase 1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Zibo Wei,Shuangyan Ye,Haipeng Feng,Chong Zeng,Xinhuai Dong,Xiaokang Zeng,Liming Zeng,Xu Lin,Qiuzhen Liu,Jie Yao
View all publications
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