AB264055

Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1]

Recombinant
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(2 Publications)

Mouse Recombinant Monoclonal IDH1 mutated R132G antibody. Suitable for ELISA, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

3 Images

ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (AB264055)

ELISA

Supplier Data

ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (AB264055)

Antigen : Human IDH1, Human IDH1(R132H), Human IDH1(R132G), Human IDH1(R132L), Human IDH1(R132S), Human IDH1(R132V), Human IDH1(R132C)).

Antigen concentration : 50 ng/ml.

ab264055 used at 0 - 1000 ng/ml.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）secondary antibody was used at 1 : 1000 dilution.

ELISA

Supplier Data

ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (AB264055)

Antigen : Human IDH2, Human IDH2(R172G), Human IDH2(R172M), Human IDH2(R172S), Human IDH2(R172W), Human IDH2(R172K), Human IDH2(R140Q).

Antigen concentration : 50 ng/ml.

ab264055 used at 0 - 1000 ng/ml.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）secondary antibody was used at 1 : 1000 dilution.

Supplier Data

Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (AB264055)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The loading samples are E.coil extracts containing recombinant protein respectively.

Exposure time : 10 seconds

All lanes:

Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (ab264055) at 1/1000 dilution

Lane 1:

His-tagged human IDH1 (aa1-414) recombinant protein at 0.01 µg

Lane 2:

His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein at 0.01 µg

Lane 3:

His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein at 0.01 µg

Lane 4:

His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein at 0.01 µg

Lane 5:

His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein at 0.01 µg

Lane 6:

His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein at 0.01 µg

Lane 7:

His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein at 0.01 µg

Lane 8:

His-tagged human IDH2 (aa40-452) recombinant protein at 0.01 µg

Lane 9:

His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein at 0.01 µg

Lane 10:

His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein at 0.01 µg

Lane 11:

His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein at 0.01 µg

Lane 12:

His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein at 0.01 µg

Lane 13:

His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein at 0.01 µg

Lane 14:

His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein at 0.01 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 46 kDa

false

Carrier free

Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MsMab-1

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody also showed the cross reactivity with other mutants of IDH1 and IDH2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.25 µg/mL", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDH1 and IDH2 also known as isocitrate dehydrogenase 1 and 2 play important roles in cellular metabolism. IDH1 has an approximate molecular mass of 45 kDa and is primarily expressed in the cytoplasm and peroxisomes whereas IDH2 with a similar mass is mainly localized in the mitochondria. These enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate coupled with the conversion of NADP+ to NADPH. This reaction contributes to cellular energy production and antioxidant defense.

Biological function summary

The IDH1 and IDH2 enzymes function by maintaining cellular redox balance and providing reductive power for biosynthesis. They also participate as part of the tricarboxylic acid (TCA) cycle where their enzymatic activity is important for the continuation of this metabolic pathway. The role of IDH1 and IDH2 within the TCA cycle ensures the efficient conversion of nutrients into energy. Their activity reflects a direct influence on central metabolic processes including lipid biosynthesis glucose metabolism and the generation of cellular building blocks.

Pathways

Both IDH1 and IDH2 are fundamental components of the TCA cycle and are also involved in the reductive carboxylation pathway. Their function involves key interactions with other metabolic proteins such as alpha-ketoglutarate dehydrogenase which also feeds into the TCA cycle. Fatty acid synthesis and glutamine metabolism pathways also align with IDH1 and IDH2 functions linking them to essential cellular processes that balance energy production with biosynthetic needs.

IDH1 and IDH2 mutations have been strongly associated with gliomas and acute myeloid leukemia (AML). These mutations significantly alter the normal enzymatic activity leading to the production of the oncometabolite 2-hydroxyglutarate. This metabolite disrupts cellular metabolism and epigenetic regulation contributing to cancer progression. Furthermore the dysfunctional IDH enzymes connect with dysfunctional TET (Ten-Eleven Translocation) proteins which are involved in DNA methylation and gene expression regulation highlighting their involvement in tumorigenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

See full target information IDH1 mutated R132G

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 478:1274-9 PubMed27553275

2016

Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Kitago,Mika K Kaneko,Satoshi Ogasawara,Yukinari Kato,Junichi Takagi

The Tohoku journal of experimental medicine 230:103-9 PubMed23782684

2013

Establishment of a multi-specific monoclonal antibody MsMab-1 recognizing both IDH1 and IDH2 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Mika Kato Kaneko,Satoshi Ogasawara,Yukinari Kato

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1]

ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (AB264055)

ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (AB264055)

Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (AB264055)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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