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AB264085

Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free

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(2 Publications)

Mouse Recombinant Monoclonal IDH1 mutated R132G antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

3 Images
ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085)
  • ELISA

Supplier Data

ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085)

This data was developed using ab264055, the same antibody clone in a different buffer formulation.

Antigen : Human IDH1, Human IDH1(R132H), Human IDH1(R132G), Human IDH1(R132L), Human IDH1(R132S), Human IDH1(R132V), Human IDH1(R132C)).

Antigen concentration : 50 ng/ml.

ab264055 used at 0 - 1000 ng/ml.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）secondary antibody was used at 1 : 1000 dilution.

ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085)
  • ELISA

Supplier Data

ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085)

This data was developed using ab264055, the same antibody clone in a different buffer formulation.

Antigen : Human IDH2, Human IDH2(R172G), Human IDH2(R172M), Human IDH2(R172S), Human IDH2(R172W), Human IDH2(R172K), Human IDH2(R140Q).

Antigen concentration : 50 ng/ml.

ab264055 used at 0 - 1000 ng/ml.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）secondary antibody was used at 1 : 1000 dilution.

Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085)
  • WB

Supplier Data

Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085)

This data was developed using ab264055, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The loading samples are E.coil extracts containing recombinant protein respectively.

Exposure time : 10 seconds

All lanes:

Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (<a href='/en-us/products/primary-antibodies/idh1-mutated-r132g-idh2-mutated-r172g-antibody-msmab-1-ab264055'>ab264055</a>) at 1/1000 dilution

Lane 1:

His-tagged human IDH1 (aa1-414) recombinant protein at 0.01 µg

Lane 2:

His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein at 0.01 µg

Lane 3:

His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein at 0.01 µg

Lane 4:

His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein at 0.01 µg

Lane 5:

His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein at 0.01 µg

Lane 6:

His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein at 0.01 µg

Lane 7:

His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein at 0.01 µg

Lane 8:

His-tagged human IDH2 (aa40-452) recombinant protein at 0.01 µg

Lane 9:

His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein at 0.01 µg

Lane 10:

His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein at 0.01 µg

Lane 11:

His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein at 0.01 µg

Lane 12:

His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein at 0.01 µg

Lane 13:

His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein at 0.01 µg

Lane 14:

His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein at 0.01 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 46 kDa

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  • Unconjugated

    Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MsMab-1

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody also showed the cross reactivity with other mutants of IDH1 and IDH2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab264085 is the carrier-free version of ab264055.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDH1 and IDH2 also known as isocitrate dehydrogenase 1 and 2 play important roles in cellular metabolism. IDH1 has an approximate molecular mass of 45 kDa and is primarily expressed in the cytoplasm and peroxisomes whereas IDH2 with a similar mass is mainly localized in the mitochondria. These enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate coupled with the conversion of NADP+ to NADPH. This reaction contributes to cellular energy production and antioxidant defense.
Biological function summary

The IDH1 and IDH2 enzymes function by maintaining cellular redox balance and providing reductive power for biosynthesis. They also participate as part of the tricarboxylic acid (TCA) cycle where their enzymatic activity is important for the continuation of this metabolic pathway. The role of IDH1 and IDH2 within the TCA cycle ensures the efficient conversion of nutrients into energy. Their activity reflects a direct influence on central metabolic processes including lipid biosynthesis glucose metabolism and the generation of cellular building blocks.

Pathways

Both IDH1 and IDH2 are fundamental components of the TCA cycle and are also involved in the reductive carboxylation pathway. Their function involves key interactions with other metabolic proteins such as alpha-ketoglutarate dehydrogenase which also feeds into the TCA cycle. Fatty acid synthesis and glutamine metabolism pathways also align with IDH1 and IDH2 functions linking them to essential cellular processes that balance energy production with biosynthetic needs.

IDH1 and IDH2 mutations have been strongly associated with gliomas and acute myeloid leukemia (AML). These mutations significantly alter the normal enzymatic activity leading to the production of the oncometabolite 2-hydroxyglutarate. This metabolite disrupts cellular metabolism and epigenetic regulation contributing to cancer progression. Furthermore the dysfunctional IDH enzymes connect with dysfunctional TET (Ten-Eleven Translocation) proteins which are involved in DNA methylation and gene expression regulation highlighting their involvement in tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).
See full target information IDH1 mutated R132G
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 478:1274-9 PubMed27553275

2016

Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Kitago,Mika K Kaneko,Satoshi Ogasawara,Yukinari Kato,Junichi Takagi

The Tohoku journal of experimental medicine 230:103-9 PubMed23782684

2013

Establishment of a multi-specific monoclonal antibody MsMab-1 recognizing both IDH1 and IDH2 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Mika Kato Kaneko,Satoshi Ogasawara,Yukinari Kato
View all publications
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