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Mouse Recombinant Monoclonal IDH1 mutated R132G antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085), expandable thumbnail
  • ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085), expandable thumbnail
  • ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (AB264085), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

Additional Targets

IDH2 mutated R172G

Alternative names

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

Recommended products

Mouse Recombinant Monoclonal IDH1 mutated R132G antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
MsMab-1
Purification technique
Affinity purification Protein A
Specificity

The antibody also showed the cross reactivity with other mutants of IDH1 and IDH2.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab264085 is the carrier-free version of Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] ab264055.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IDH1 and IDH2 also known as isocitrate dehydrogenase 1 and 2 play important roles in cellular metabolism. IDH1 has an approximate molecular mass of 45 kDa and is primarily expressed in the cytoplasm and peroxisomes whereas IDH2 with a similar mass is mainly localized in the mitochondria. These enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate coupled with the conversion of NADP+ to NADPH. This reaction contributes to cellular energy production and antioxidant defense.

Biological function summary

The IDH1 and IDH2 enzymes function by maintaining cellular redox balance and providing reductive power for biosynthesis. They also participate as part of the tricarboxylic acid (TCA) cycle where their enzymatic activity is important for the continuation of this metabolic pathway. The role of IDH1 and IDH2 within the TCA cycle ensures the efficient conversion of nutrients into energy. Their activity reflects a direct influence on central metabolic processes including lipid biosynthesis glucose metabolism and the generation of cellular building blocks.

Pathways

Both IDH1 and IDH2 are fundamental components of the TCA cycle and are also involved in the reductive carboxylation pathway. Their function involves key interactions with other metabolic proteins such as alpha-ketoglutarate dehydrogenase which also feeds into the TCA cycle. Fatty acid synthesis and glutamine metabolism pathways also align with IDH1 and IDH2 functions linking them to essential cellular processes that balance energy production with biosynthetic needs.

Associated diseases and disorders

IDH1 and IDH2 mutations have been strongly associated with gliomas and acute myeloid leukemia (AML). These mutations significantly alter the normal enzymatic activity leading to the production of the oncometabolite 2-hydroxyglutarate. This metabolite disrupts cellular metabolism and epigenetic regulation contributing to cancer progression. Furthermore the dysfunctional IDH enzymes connect with dysfunctional TET (Ten-Eleven Translocation) proteins which are involved in DNA methylation and gene expression regulation highlighting their involvement in tumorigenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (ab264085), expandable thumbnail

    Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (ab264085)

    This data was developed using Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] ab264055, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The loading samples are E.coil extracts containing recombinant protein respectively.

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] ab264055) at 1/1000 dilution

    Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein at 0.01 µg

    Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein at 0.01 µg

    Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein at 0.01 µg

    Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein at 0.01 µg

    Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein at 0.01 µg

    Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein at 0.01 µg

    Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein at 0.01 µg

    Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein at 0.01 µg

    Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein at 0.01 µg

    Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein at 0.01 µg

    Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein at 0.01 µg

    Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein at 0.01 µg

    Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein at 0.01 µg

    Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein at 0.01 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 46 kDa

  • ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (ab264085), expandable thumbnail

    ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (ab264085)

    This data was developed using Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] ab264055, the same antibody clone in a different buffer formulation.

    Antigen: Human IDH1, Human IDH1(R132H), Human IDH1(R132G), Human IDH1(R132L), Human IDH1(R132S), Human IDH1(R132V), Human IDH1(R132C)).

    Antigen concentration: 50 ng/ml.

    Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] ab264055 used at 0 - 1000 ng/ml.

    Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）secondary antibody was used at 1:1000 dilution.

  • ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (ab264085), expandable thumbnail

    ELISA - Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] - BSA and Azide free (ab264085)

    This data was developed using Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] ab264055, the same antibody clone in a different buffer formulation.

    Antigen: Human IDH2, Human IDH2(R172G), Human IDH2(R172M), Human IDH2(R172S), Human IDH2(R172W), Human IDH2(R172K), Human IDH2(R140Q).

    Antigen concentration: 50 ng/ml.

    Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] ab264055 used at 0 - 1000 ng/ml.

    Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L）secondary antibody was used at 1:1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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