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Mouse Recombinant Monoclonal IDH1 mutated R132S antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-IDH1 (mutated R132S) antibody [SMab-1] - BSA and Azide free (AB264057), expandable thumbnail
  • Indirect ELISA - Anti-IDH1 (mutated R132S) antibody [SMab-1] - BSA and Azide free (AB264057), expandable thumbnail

Publications

  • Biochemical and biophysical research communication 406:608-132011
    Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Mika Kato Kaneko et. al.
    PubMed 21352804

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBI-ELISA
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Alternative names

    Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, IDH1

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    Mouse Recombinant Monoclonal IDH1 mutated R132S antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Human samples. Cited in 1 publication.

    Alternative names

    Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, IDH1

    Key facts

    Isotype

    IgG1

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    SMab-1

    Purification technique

    Affinity purification Protein A

    Light chain type

    kappa

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Storage information

    Do Not Freeze

    Notes

    ab264057 is the carrier-free version of Anti-IDH1 (mutated R132S) antibody [SMab-1] ab264049.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    IDH1 also known as isocitrate dehydrogenase 1 is an enzyme with a molecular weight of approximately 45 kDa. It participates in the citric acid cycle aiding the conversion of isocitrate to alpha-ketoglutarate while producing NADPH. IDH1 resides primarily in the cytoplasm and peroxisomes of cells. It finds expression in various tissues most notably the liver and brain where metabolic processes are highly active.

    Biological function summary

    Isocitrate dehydrogenase 1 plays an important role in cellular metabolism by helping to maintain the NADP+/NADPH balance important for redox reactions. This enzyme operates mainly as a homodimer meaning two identical subunits form a complex for functional activity. It contributes significantly to lipid and glucose metabolism due to its production of NADPH which the cells use for biosynthetic pathways and detoxification.

    Pathways

    The involvement of IDH1 in the citric acid cycle highlights its importance in energy production and metabolic regulation. This enzyme closely associates with other metabolic enzymes such as IDH2 another member of the isocitrate dehydrogenase family found in mitochondria suggesting a coordinated function between cytoplasmic and mitochondrial metabolism. Beyond its role in the citric acid cycle IDH1 also relates to the pentose phosphate pathway an alternative glucose metabolism route providing reducing equivalents and ribose 5-phosphate for nucleotide synthesis.

    Associated diseases and disorders

    IDH1 mutations particularly the IDH1 R132H variant frequently appear in certain cancers like gliomas and acute myeloid leukemia (AML). These mutations lead to a neomorphic enzyme function producing 2-hydroxyglutarate instead of alpha-ketoglutarate disrupting normal cellular metabolism and aiding tumorigenesis. The mutated IDH1 also affects the TET2 protein which involves in DNA demethylation potentially altering gene expression and contributing to oncogenic pathways.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    2 product images

    • Western blot - Anti-IDH1 (mutated R132S) antibody [SMab-1] - BSA and Azide free (ab264057), expandable thumbnail

      Western blot - Anti-IDH1 (mutated R132S) antibody [SMab-1] - BSA and Azide free (ab264057)

      Anti-IDH1 (mutated R132S) antibody [SMab-1] ab264049 used at 0.949μg/ml.
      Goat Anti-Rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) used as the secondary at a 1/10,000 dilution.
      Blocking/Dilution buffer:5% NFDM/TBST.

      Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
      Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
      Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
      Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
      Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
      Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
      Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
      Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
      Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
      Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
      Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
      Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
      Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
      Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

      The loading samples are E.coil extracts containing recombinant protein respectively.
      Exposure time of 3 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IDH1 (mutated R132S) antibody [SMab-1] ab264049).

      All lanes: Western blot - Anti-IDH1 (mutated R132S) antibody [SMab-1] (Anti-IDH1 (mutated R132S) antibody [SMab-1] ab264049)

      Predicted band size: 46 kDa

    • Indirect ELISA - Anti-IDH1 (mutated R132S) antibody [SMab-1] - BSA and Azide free (ab264057), expandable thumbnail

      Indirect ELISA - Anti-IDH1 (mutated R132S) antibody [SMab-1] - BSA and Azide free (ab264057)

      ELISA - Anti-IDH1 (mutated R132 S) antibody [SMab-1].

      Anti-IDH1 (mutated R132S) antibody [SMab-1] ab264049 used at 0-5000 ng/ml.

      Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.

      Antigen concentration 1000 ng/ml.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IDH1 (mutated R132S) antibody [SMab-1] ab264049).

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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