AB177512

Anti-IDH2 antibody

Name: Anti-IDH2 antibody (ab177512) | Abcam
Brand: Abcam Limited
SKU: AB177512
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal IDH2 antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IDH2 aa 400 to C-terminus.

View Alternative Names

IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2

4 Images

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Immunocytochemistry - Anti-IDH2 antibody (AB177512)

Immunocytochemistry analysis of HeLa cells labelling IDH2 with ab177512 at 1 μg/mL. Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated was used as a secondary antivody at a 1/100 dilution.

Supplier Data

Immunoprecipitation - Anti-IDH2 antibody (AB177512)

Lane 1 : ab177512 at 1μg/ml labeling IDH2 in 293T whole cell lysate immunoprecipitated using ab177512 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-IDH2 antibody (ab177512)

Predicted band size: 50 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody (AB177512)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody (AB177512)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling IDH2 with ab177512 at 1 μg/mL.

Detection : DAB

Supplier Data

Western blot - Anti-IDH2 antibody (AB177512)

All lanes:

Western blot - Anti-IDH2 antibody (ab177512) at 0.4 µg/mL

Lane 1:

Whole cell lysate from 293T cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 50 µg

Lane 3:

Whole cell lysate from Jurkat cells at 50 µg

Lane 4:

Whole cell lysate from TCMK-1 cells at 50 µg

Lane 5:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC, IP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human IDH2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P48735

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.

Biological function summary

The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.

Pathways

The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.

The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in intermediary metabolism and energy production (PubMed : 19228619, PubMed : 22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed : 19228619, PubMed : 22416140).

See full target information IDH2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37446160

2023

Cryopreservation Induces Acetylation of Metabolism-Related Proteins in Boar Sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Malik Ahsan Ali,Ziyue Qin,Shan Dou,Anqi Huang,Yihan Wang,Xiang Yuan,Yan Zhang,Qingyong Ni,Rameesha Azmat,Changjun Zeng

Journal of experimental & clinical cancer research : CR 41:261 PubMed36028854

2022

ALKBH5-mediated m6A modification of circCCDC134 facilitates cervical cancer metastasis by enhancing HIF1A transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Leilei Liang,Yunshu Zhu,Jian Li,Jia Zeng,Lingying Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IDH2 antibody

Immunocytochemistry - Anti-IDH2 antibody (AB177512)

Immunoprecipitation - Anti-IDH2 antibody (AB177512)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody (AB177512)

Western blot - Anti-IDH2 antibody (AB177512)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise