Knockout Tested Rabbit Recombinant Monoclonal IDH2 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--western-blot-img175874.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343)")
![Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--western-blot-img6874.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122391.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343)")
![Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--flow-cytometry-intracellular-img26624.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343)")
![Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--western-blot-img435385.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (AB246343)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested |
| Mouse | Expected | Not recommended | Expected | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Plays a role in intermediary metabolism and energy production (PubMed:19228619, PubMed:22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed:19228619, PubMed:22416140).
Alternative names
IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal IDH2 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7576
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab246343 is the carrier-free version of Anti-IDH2 antibody [EPR7576] ab129180.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.
- Biological function summary
The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.
- Pathways
The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.
- Associated diseases and disorders
The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--western-blot-img175874.jpg "Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)")
Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)
False colour image of Western blot: Anti-IDH2 antibody [EPR7576] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-IDH2 antibody [EPR7576] ab129180 was shown to bind specifically to IDH2. A band was observed at 48 kDa in wild-type Jurkat cell lysates with no signal observed at this size in IDH2 knockout cell line Human IDH2 knockout Jurkat cell line ab282331 (knockout cell lysate ab283148). To generate this image, wild-type and IDH2 knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-IDH2 antibody [EPR7576] (Anti-IDH2 antibody [EPR7576] ab129180) at 1/1000 dilution
Lane 1: Wild-type Jurkat cell lysate at 20 µg
Lane 2: IDH2 knockout Jurkat cell lysate at 20 µg
Lane 2: Western blot - Human IDH2 knockout Jurkat cell line (Human IDH2 knockout Jurkat cell line ab282331)
Lane 3: MOLT-4 cell lysate at 20 µg
Lane 4: HEK-293 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 48 kDa
![Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--western-blot-img6874.jpg "Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)")
Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: IDH2 knockout HAP1 cell lysate (20 μg)
Lane 3: K562 cell lysate (20 μg)
Lane 4: HepG2 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-IDH2 antibody [EPR7576] ab129180 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-IDH2 antibody [EPR7576] ab129180 was shown to recognize IDH2 when IDH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and IDH2 knockout samples were subjected to SDS-PAGE. Anti-IDH2 antibody [EPR7576] ab129180 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IDH2 antibody [EPR7576] ab129180).
All lanes: Western blot - Anti-IDH2 antibody [EPR7576] (Anti-IDH2 antibody [EPR7576] ab129180)
Predicted band size: 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122391.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)
Anti-IDH2 antibody [EPR7576] ab129180, at 1/250 dilution, staining IDH2 in Formalin-fixed, Paraffin-embedded Human thyroid gland carcinoma by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IDH2 antibody [EPR7576] ab129180).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--flow-cytometry-intracellular-img26624.jpg "Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)")
Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)
Overlay histogram showing MCF7 cells stained with Anti-IDH2 antibody [EPR7576] ab129180 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-IDH2 antibody [EPR7576] ab129180, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IDH2 antibody [EPR7576] ab129180).
![Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343), expandable thumbnail](https://content.abcam.com/products/images/idh2-antibody-epr7576-bsa-and-azide-free-ab246343--western-blot-img435385.jpg "Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)")
Western blot - Anti-IDH2 antibody [EPR7576] - BSA and Azide free (ab246343)
This data was developed using the same antibody clone in a different buffer formulation (abAB129180).
Western blot: Anti-IDH2 antibody [EPR7576] (Anti-IDH2 antibody [EPR7576] ab129180) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-IDH2 antibody [EPR7576] ab129180 was shown to bind specifically to IDH2. A band was observed at 51 kDa in wild-type A549 cell lysates with no signal observed at this size in IDH2 knockout cell line. To generate this image, wild-type and IDH2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-IDH2 antibody [EPR7576] (Anti-IDH2 antibody [EPR7576] ab129180) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: IDH2 knockout A549 cell lysate at 20 µg
Lane 3: Wild-type Jurkat cell lysate at 20 µg
Lane 4: IDH2 knockout Jurkat Human IDH2 knockout Jurkat cell line ab282331 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 51 kDa
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