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Anti-IDH2 antibody [EPR7577] (ab131263) is a rabbit monoclonal antibody that is used to detect IDH2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with IDH2 knockout cell line validation


Images

Western blot - Anti-IDH2 antibody [EPR7577] (AB131263), expandable thumbnail
  • Western blot - Anti-IDH2 antibody [EPR7577] (AB131263), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7577] (AB131263), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IDH2 antibody [EPR7577] (AB131263), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (AB131263), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Rat
Tested
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/1000
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Plays a role in intermediary metabolism and energy production (PubMed:19228619, PubMed:22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed:19228619, PubMed:22416140).

Alternative names

IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2

Recommended products

Anti-IDH2 antibody [EPR7577] (ab131263) is a rabbit monoclonal antibody that is used to detect IDH2 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with IDH2 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7577
Purification technique
Affinity purification Protein A
Dissociation constant
4.7 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-IDH2 antibody [EPR7577] (ab131263) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of IDH2?


Anti-IDH2 [EPR7577] (ab131263) specifically detects a band for IDH2 (UniProt: P48735) at a molecular weight of 50kDa.

Recommended positive controls


Wild-type A549, Wild-type Jurkat, MOLT-4, K562, U-87 MG, and HepG2 cell lysates.

Trusted by the scientific community


Anti-IDH2 [EPR7577] (ab131263) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-IDH2 antibody [EPR7577] (ab131263) has been confirmed by Western blot testing in IDH2 Knockout HAP1 cell line, Human IDH2 knockout Jurkat cell line ab282331.



Other related products


We have a range of other formats of antibody clone [EPR7577] also available for your convenience:
ab131263, PE - PE Anti-IDH2 antibody [EPR7577] ab212122, Carrier free - Anti-IDH2 antibody [EPR7577] - BSA and Azide free ab230796, APC - APC Anti-IDH2 antibody [EPR7577] ab317932, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-IDH2 antibody [EPR7577] ab318055



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.

Biological function summary

The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.

Pathways

The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.

Associated diseases and disorders

The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

  • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)

    False colour image of Western blot: Anti-IDH2 antibody [EPR7577] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab131263 was shown to bind specifically to IDH2. A band was observed at 48 kDa in wild-type Jurkat cell lysates with no signal observed at this size in IDH2 knockout cell line Human IDH2 knockout Jurkat cell line ab282331 (knockout cell lysate ab283148). To generate this image, wild-type and IDH2 knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-IDH2 antibody [EPR7577] (ab131263) at 1/1000 dilution

    Lane 1: Wild-type Jurkat cell lysate at 20 µg

    Lane 2: IDH2 knockout Jurkat cell lysate at 20 µg

    Lane 2: Western blot - Human IDH2 knockout Jurkat cell line (Human IDH2 knockout Jurkat cell line ab282331)

    Performed under reducing conditions.

    Predicted band size: 50 kDa

    Observed band size: 48 kDa

  • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)

    All lanes: Western blot - Anti-IDH2 antibody [EPR7577] (ab131263) at 1/5000 dilution

    Lane 1: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: Mouse liver lysate at 20 µg

    Lane 6: Rat liver lysate at 20 µg

    Lane 7: Mouse kidney lysate at 20 µg

    Lane 8: Rat kidney lysate at 20 µg

    Lane 9: Rat stomach lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa

  • Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IDH2 antibody [EPR7577] (ab131263)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling IDH2 with Purified ab131263 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IDH2 antibody [EPR7577] (ab131263)

    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling IDH2 with Purified ab131263 at 1:1000 dilution (0.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling IDH2 with Purified ab131263 at 1:100 (2.11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling IDH2 with Purified ab131263 at 1:100 (2.11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: IDH2 knockout HAP1 cell lysate (20 μg)
    Lane 3: K562 cell lysate (20 μg)
    Lane 4: HepG2 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab131263 observed at 48 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab131263 was shown to recognize IDH2 when IDH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and IDH2 knockout samples were subjected to SDS-PAGE. ab131263 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling IDH2 with Purified ab131263 at 1:100 (2.11 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263), expandable thumbnail

    Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)

    Western blot: Anti-IDH2 antibody [EPR7577] (ab131263) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab131263 was shown to bind specifically to IDH2. A band was observed at 51 kDa in wild-type A549 cell lysates with no signal observed at this size in IDH2 knockout cell line. To generate this image, wild-type and IDH2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-IDH2 antibody [EPR7577] (ab131263) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: IDH2 knockout A549 cell lysate at 20 µg

    Lane 3: Wild-type Jurkat cell lysate at 20 µg

    Lane 4: IDH2 knockout Jurkat Human IDH2 knockout Jurkat cell line ab282331 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 51 kDa

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