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AB284664

Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] - BSA and Azide free

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Rat Recombinant Monoclonal IDH2 mutated R172K antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Recombinant full length protein - Human, Synthetic peptide - Human samples.

View Alternative Names

IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2

2 Images
Western blot - Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] - BSA and Azide free (AB284664)
  • WB

Lab

Western blot - Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] - BSA and Azide free (AB284664)

This data was developed using ab264052, the same antibody clone in a different buffer formulation.

ab264052 used at 1/1000 dilution (0.805 μg/ml).

Secondary used : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10,000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

The loading samples are E.coil extracts containing recombinant protein respectively.

Lane 1 : His-tagged human IDH1 (aa1-414) recombinant protein 10 ng

Lane 2 : His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10 ng

Lane 3 : His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10 ng

Lane 4 : His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10 ng

Lane 5 : His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10 ng

Lane 6 : His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10 ng

Lane 7 : His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10 ng

Lane 8 : His-tagged human IDH2 (aa40-452) recombinant protein 10 ng

Lane 9 : His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10 ng

Lane 10 : His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10 ng

Lane 11 : His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10 ng

Lane 12 : His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10 ng

Lane 13 : His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10 ng

Lane 14 : His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10 ng

All lanes:

Western blot - Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (<a href='/en-us/products/primary-antibodies/idh2-mutated-r172k-antibody-r511-kmab-1-ab264052'>ab264052</a>)

Predicted band size: 50 kDa

false

Exposure time: 15s

Indirect ELISA - Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] - BSA and Azide free (AB284664)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] - BSA and Azide free (AB284664)

This data was developed using ab264052, the same antibody clone in a different buffer formulation.ab264052 used at 0-5000 ng/ml.

Antigen (IDH2, IDH2(R172G), IDH2(R172M), IDH2(R172S), IDH2(R172W), IDH2(R172K), IDH2(R140Q)) used at 100 ng/ml.

Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

  • Unconjugated

    Anti-IDH2 (mutated R172K) antibody [R511-KMab-1]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

R511-KMab-1

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Primary Antibodies

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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Product details

ab284664 is the carrier-free version of ab264052

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.
Biological function summary

The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.

Pathways

The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.

The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in intermediary metabolism and energy production (PubMed : 19228619, PubMed : 22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed : 19228619, PubMed : 22416140).
See full target information IDH2 R172K
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com