AB264053

Anti-IDH2 (mutated R172M) antibody [MMab-1]

Recombinant
What is this?

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(1 Publication)

Rat Recombinant Monoclonal IDH2 mutated R172M antibody. Suitable for WB, I-ELISA and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2

2 Images

Lab

Western blot - Anti-IDH2 (mutated R172M) antibody [MMab-1] (AB264053)

ab264053 used at 0.978ug/ml, followed by Goat Anti-Rat IgG (H+L), HRP) (ab205720) secondary used at a 1/10,000 dilution.
Blocking/Dilution buffer : 5% NFDM/TBST

Lane 1 : His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2 : His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3 : His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4 : His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5 : His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6 : His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7 : His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8 : His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9 : His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10 : His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11 : His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12 : His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13 : His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14 : His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

The loading samples are E.coil extracts containing recombinant protein respectively.
Exposure time of 70 seconds.

All lanes:

Western blot - Anti-IDH2 (mutated R172M) antibody [MMab-1] (ab264053)

Predicted band size: 50 kDa

false

Indirect ELISA - Anti-IDH2 (mutated R172M) antibody [MMab-1] (AB264053)

I-ELISA

Lab

Indirect ELISA - Anti-IDH2 (mutated R172M) antibody [MMab-1] (AB264053)

ELISA - Anti-IDH2 (mutated R172 M) antibody [MMab-1].

ab264053 used at 0-5000 ng/ml.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used as the secondary at a 1/1000 dilution.

Antigen concentration 100 ng/ml.

Carrier free

Anti-IDH2 (mutated R172M) antibody [MMab-1] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MMab-1

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.978 µg/mL", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "3.135 µg/mL", "IELISA-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.

Biological function summary

The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.

Pathways

The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.

The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in intermediary metabolism and energy production (PubMed : 19228619, PubMed : 22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed : 19228619, PubMed : 22416140).

See full target information IDH2 mutated R172M

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 432:40-5 PubMed23376717

2013

Establishment of novel monoclonal antibodies KMab-1 and MMab-1 specific for IDH2 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Mika Kato Kaneko,Shunpei Morita,Yuta Tsujimoto,Ryo Yanagiya,Kana Nasu,Hiroko Sasaki,Yasukazu Hozumi,Kaoru Goto,Atsushi Natsume,Mika Watanabe,Toshihiro Kumabe,Shingo Takano,Yukinari Kato

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IDH2 (mutated R172M) antibody [MMab-1]

Western blot - Anti-IDH2 (mutated R172M) antibody [MMab-1] (AB264053)

Indirect ELISA - Anti-IDH2 (mutated R172M) antibody [MMab-1] (AB264053)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise