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AB264056

Anti-IDH2 (mutated R172S) antibody [SMab-2]

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(1 Publication)

Mouse Recombinant Monoclonal IDH2 mutated R172S antibody. Suitable for WB, I-ELISA, ICC/IF and reacts with Recombinant fragment - Human, Human, Synthetic peptide - Human samples. Cited in 1 publication.

View Alternative Names

IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IDH2 (mutated R172S) antibody [SMab-2] (AB264056)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IDH2 (mutated R172S) antibody [SMab-2] (AB264056)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW1353 (human chondrosarcoma cell line) cells labelling IDH2 (mutated R172 S) with ab264056 at 1/50 dilution (26.58μg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SW1353 cells.

Negative control : HeLa (PMID : 25753205).

ab179504 Anti-beta IV Tubulin antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab264056 at a 1/50 dilution (26.58μg/ml) followed by ab150080 at a 1/1000 dilution.

Negative control 2 : ab179504 at 1/200 dilution followed by ab150113 at a 1/1000 dilution.

Western blot - Anti-IDH2 (mutated R172S) antibody [SMab-2] (AB264056)
  • WB

Lab

Western blot - Anti-IDH2 (mutated R172S) antibody [SMab-2] (AB264056)

Western blot - Anti-IDH2 (mutated R172 S) antibody [SMab-2] (ab264056).

The loading samples are E.coil extracts containing recombinant protein respectively.

ab264056 used at a 1/1000 dilution (1.329μg/ml), followed by Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) secondary antibody at a 1/10,000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

Lane 1 : His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2 : His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3 : His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4 : His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5 : His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6 : His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7 : His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8 : His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9 : His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10 : His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11 : His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12 : His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13 : His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14 : His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

All lanes:

Western blot - Anti-IDH2 (mutated R172S) antibody [SMab-2] (ab264056)

Predicted band size: 50 kDa

false

Exposure time: 3min

Indirect ELISA - Anti-IDH2 (mutated R172S) antibody [SMab-2] (AB264056)
  • I-ELISA

Lab

Indirect ELISA - Anti-IDH2 (mutated R172S) antibody [SMab-2] (AB264056)

ELISA - Anti-IDH2 (mutated R172 S) antibody [SMab-2] (ab264056).

ab264056 used at 0-5000 ng/ml, followed by an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) secondary used at 1/1000 dilution.

Antigen concentration, 100 ng/ml.

  • Carrier free

    Anti-IDH2 (mutated R172S) antibody [SMab-2] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SMab-2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "26.58 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1.329 µg/mL", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Use at 1250ng/ml.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.
Biological function summary

The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.

Pathways

The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.

The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in intermediary metabolism and energy production (PubMed : 19228619, PubMed : 22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed : 19228619, PubMed : 22416140).
See full target information IDH2 mutated R172S
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 459:636-42 PubMed25753205

2015

A novel monoclonal antibody SMab-2 recognizes endogenous IDH2-R172S of chondrosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Liu,Satoshi Ogasawara,Mika K Kaneko,Hiroharu Oki,Yasukazu Hozumi,Kaoru Goto,Michiaki Takagi,Yukinari Kato
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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