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AB264062

Anti-IDH2 (mutated R172W) antibody [WMab-1] - BSA and Azide free

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(1 Publication)

Rat Recombinant Monoclonal IDH2 mutated R172W antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IDH, ICD-M, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH2

2 Images
Western blot - Anti-IDH2 (mutated R172W) antibody [WMab-1] - BSA and Azide free (AB264062)
  • WB

Lab

Western blot - Anti-IDH2 (mutated R172W) antibody [WMab-1] - BSA and Azide free (AB264062)

ab264054 used at 1.413ug/ml, followed by Goat Anti-Rat IgG (H+L), HRP) (ab205720) secondary used at a 1/10,000 dilution.

Lane 1 : His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2 : His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3 : His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4 : His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5 : His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6 : His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7 : His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8 : His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9 : His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10 : His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11 : His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12 : His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13 : His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14 : His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

The loading samples are E.coil extracts containing recombinant protein respectively.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time of 70 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264054).

All lanes:

Western blot - Anti-IDH2 (mutated R172W) antibody [WMab-1] (<a href='/en-us/products/primary-antibodies/idh2-mutated-r172w-antibody-wmab-1-ab264054'>ab264054</a>)

Predicted band size: 50 kDa

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Indirect ELISA - Anti-IDH2 (mutated R172W) antibody [WMab-1] - BSA and Azide free (AB264062)
  • I-ELISA

Lab

Indirect ELISA - Anti-IDH2 (mutated R172W) antibody [WMab-1] - BSA and Azide free (AB264062)

ELISA - Anti-IDH2 (mutated R172 W) antibody [WMab-1].

ab264054 used at 0-5000ng/ml.

Antigen concentration is 100ng/ml.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used as the secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264054).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

WMab-1

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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Product details

ab264062 is the carrier-free version of ab264054.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Isocitrate dehydrogenase 2 (IDH2) is an enzyme that converts isocitrate to alpha-ketoglutarate in the citric acid cycle through oxidative decarboxylation. IDH2 also known as NADP-dependent isocitrate dehydrogenase has a molecular weight of about 51 kDa. This protein expresses in the mitochondria serving an important role in cellular energy production and intermediary metabolism. By facilitating this conversion IDH2 impacts cellular respiration and energy balance.
Biological function summary

The IDH2 enzyme facilitates cellular metabolism by producing NADPH critical for biosynthesis and antioxidant defense. It functions within the oxidative decarboxylation of isocitrate providing reducing equivalents to keep cellular redox balance. Although not part of a larger enzymatic complex IDH2 acts synergistically with other metabolic enzymes to maintain cellular biochemical pathways.

Pathways

The IDH2 protein participates in the citric acid cycle and redox homeostasis. IDH2 contributes to the tricarboxylic acid (TCA) pathway coupling with other TCA components such as citrate synthase and aconitase. Within the redox pathway it influences glucose metabolism via its link with enzymes like NADPH oxidase ensuring a steady supply of NADPH for biosynthetic reactions.

The IDH2 protein links to certain cancers like acute myeloid leukemia (AML) due to mutations such as IDH2 R140Q and IDH2 R172K which lead to the production of oncometabolite 2-hydroxyglutarate. This oncometabolite influences epigenetic regulation and cellular differentiation. IDH2 mutations often associate with altered tumor suppressor pathways involving proteins like TP53 contributing to tumorigenesis and impaired cellular differentiation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in intermediary metabolism and energy production (PubMed : 19228619, PubMed : 22416140). It may tightly associate or interact with the pyruvate dehydrogenase complex (PubMed : 19228619, PubMed : 22416140).
See full target information IDH2 mutated R172W
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communication 433:374-8 PubMed23524262

2013

Generation of a novel monoclonal antibody WMab-1 specific for IDH2-R172W mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Yukinari Kato,Mika Kato Kaneko
View all publications
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