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AB247089

Anti-IDH3B antibody

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(1 Publication)

Rabbit Polyclonal IDH3B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial aa 50-200.

View Alternative Names

Isocitric dehydrogenase subunit beta, NAD(+)-specific ICDH subunit beta, IDH3B

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-IDH3B antibody (AB247089)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IDH3B antibody (AB247089)

PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for IDH3B (green) using ab247089 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)

Formalin-fixed, paraffin-embedded human testis tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)

Formalin-fixed, paraffin-embedded human kidney tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)

Formalin-fixed, paraffin-embedded human placenta tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)

Formalin-fixed, paraffin-embedded human parathyroid gland tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (AB247089)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

Western blot - Anti-IDH3B antibody (AB247089)
  • WB

Supplier Data

Western blot - Anti-IDH3B antibody (AB247089)

All lanes:

Western blot - Anti-IDH3B antibody (ab247089) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human rain glioma cell line) cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

O43837

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDH3B also known as isocitrate dehydrogenase 3 (NAD(+)) beta subunit is an enzyme that plays a critical role in the tricarboxylic acid (TCA) cycle. It catalyzes the conversion of isocitrate to alpha-ketoglutarate coupling this with the reduction of NAD+ to NADH. IDH3B has a molecular weight of approximately 41 kDa. Expression of IDH3B is found predominantly in the mitochondria of most eukaryotic cells including in tissues with high energy demands such as muscle and heart.
Biological function summary

IDH3B functions as a part of the IDH3 enzyme complex which consists of alpha beta and gamma subunits. This complex is essential for regulating the TCA cycle affecting cellular energy metabolism. Through its enzymatic activity IDH3B contributes to maintaining proper levels of NADH which in turn influences ATP production and overall cellular respiration.

Pathways

IDH3B serves a significant role in the TCA cycle and is interconnected with the glutamine metabolism pathway. By converting isocitrate to alpha-ketoglutarate it interfaces with proteins such as IDH3A and IDH3G which are subunits of the same enzyme complex. These interactions ensure a well-regulated flow of metabolites necessary for energy production and reductive biosynthesis.

Mutations or dysregulation of IDH3B have been linked to certain types of cancer and neurological disorders. For example some studies have shown alterations in IDH3B expression in gliomas affecting cellular metabolism and growth. Additionally IDH3B interacts with IDH1 and IDH2 which are other isocitrate dehydrogenases known for their mutated forms in various cancers. Understanding these relationships can provide insights into potential therapeutic avenues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a structural role to facilitate the assembly and ensure the full activity of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
See full target information Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:4298-4323 PubMed39160272

2024

CHC22 clathrin recruitment to the early secretory pathway requires two-site interaction with SNX5 and p115.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua Greig,George T Bates,Daowen I Yin,Kit Briant,Boris Simonetti,Peter J Cullen,Frances M Brodsky
View all publications
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