JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB224210

Anti-IDH3G antibody

5

(1 Review)

|

(1 Publication)

Rabbit Polyclonal IDH3G antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IDH3G aa 250 to C-terminus.

View Alternative Names

Isocitric dehydrogenase subunit gamma, NAD(+)-specific ICDH subunit gamma, IDH3G

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3G antibody (AB224210)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3G antibody (AB224210)

Paraffin embedded human cerebellum tissue stained for IDH3G with ab224210 (1/200 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-IDH3G antibody (AB224210)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IDH3G antibody (AB224210)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for IDH3G (green) using ab224210 (4 μg/ml) in ICC/IF.

Western blot - Anti-IDH3G antibody (AB224210)
  • WB

Supplier Data

Western blot - Anti-IDH3G antibody (AB224210)

All lanes:

Western blot - Anti-IDH3G antibody (ab224210) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 43 kDa

false

Western blot - Anti-IDH3G antibody (AB224210)
  • WB

Supplier Data

Western blot - Anti-IDH3G antibody (AB224210)

All lanes:

Western blot - Anti-IDH3G antibody (ab224210) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human IDH3G aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P51553

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IDH3G protein also called isocitrate dehydrogenase 3 (NAD+) gamma is a subunit of the IDH3 enzyme complex. This protein has a molecular mass of approximately 39 kDa. It is highly expressed in tissues with a high energy demand like muscle cells and neurons. IDH3G plays an important role in the mitochondrial matrix where it catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate producing NADH and CO2 as byproducts.
Biological function summary

IDH3G is part of the IDH3 enzyme complex which contains multiple subunits and functions within mitochondria. This complex plays a critical role in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. This cycle is fundamental for energy production as it supplies reducing equivalents in the form of NADH which drive ATP synthesis through oxidative phosphorylation. The proper functioning of IDH3G contributes to efficient cellular respiration and energy homeostasis.

Pathways

The function of IDH3G connects to the TCA cycle and the energy metabolism pathway. Within the TCA cycle IDH3G's enzymatic activity ensures the continuation of the cycle and maintains the production of NADH. This is key for the generation of ATP in oxidative phosphorylation. The IDH3 complex works closely with the other components of the TCA cycle including succinate dehydrogenase and citrate synthase ensuring the continuity and regulation of metabolic flux in energy production pathways.

Defects or alterations in IDH3G are associated with metabolic disorders such as lactic acidosis and mitochondrial diseases. These conditions can result from impaired energy production and accumulation of metabolic intermediates. Furthermore IDH3G connections with related proteins could affect cell proliferation and survival in neurodegenerative diseases. Proper regulation and function of IDH3G may impact these diseases through its influence on energy metabolism pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
See full target information IDH3G
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70464 PubMed40985995

2025

CD103CD8 tissue-resident memory T lymphocytes of melanoma boost anti-tumour immunity and predict immunotherapy outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Zhang,Junquan Song,Yinlam Li,Kangjie Shen,Jiangying Xuan,Yuan Gao,Lili Lu,Zhi Pang,Lu Wang,Yang Yang,Zixu Gao,Qianrong Hu,Yu Zhu,Chenlu Wei,Shaoluan Zheng,Rongkui Luo,Yingyong Hou,Yuhong Zhou,Chuanyuan Wei,Jianying Gu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB118289

Anti-IDH3G antibody

Western blot - Anti-IDH3G antibody (AB118289)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB110321

Anti-Aconitase 2 antibody [6F12BD9]

BOND RX™ Validated|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6F12BD9] (AB110321)

  • 5
  • 4
Primary Antibodies

AB117787

Anti-ZFP64 antibody

Immunoprecipitation - Anti-ZFP64 antibody (AB117787)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB117756

Anti-ZBTB2 antibody

Immunoprecipitation - Anti-ZBTB2 antibody (AB117756)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com