Rabbit Polyclonal IDI1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human IDI1 aa 150 to C-terminus.
View Alternative Names
Isopentenyl-diphosphate Delta-isomerase 1, Isopentenyl pyrophosphate isomerase 1, IPP isomerase 1, IPPI1, IDI1
- IP
Supplier Data
Immunoprecipitation - Anti-IDI1 antibody (AB242049)
IDI1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242049 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab242049 at 1 μg/mL.
Lane 1 : ab242049 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.
Detection : Chemiluminescence with an exposure time of 30 seconds.
Lysates prepared in NETN buffer.
All lanes:
Immunoprecipitation - Anti-IDI1 antibody (ab242049)
Predicted band size: 26 kDa
false
- WB
Supplier Data
Western blot - Anti-IDI1 antibody (AB242049)
Lysates prepared in NETN buffer.
All lanes:
Western blot - Anti-IDI1 antibody (ab242049) at 0.4 µg/mL
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Predicted band size: 26 kDa
true
Exposure time: 30s
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Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IDI1 contributes to the formation of isoprenoids which are essential for numerous cellular functions like cholesterol synthesis and protein prenylation. It principally operates as a monomer and does not typically associate with a larger protein complex during its activity. The enzyme's role in positioning alongside other essential enzymes makes it significant for the transcriptional regulation of certain metabolic processes.
Pathways
IDI1 participates extensively in the mevalonate pathway a fundamental route for cholesterol biosynthesis. It functions under the regulation of proteins like HMG-CoA reductase a rate-limiting enzyme in this pathway. The enzyme also connects to the isoprenoid biosynthesis pathway where it interacts with proteins involved in the synthesis of farnesyl pyrophosphate a precursor in the generation of various biomolecules.
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Target data
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