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AB205617

Anti-IDI1 + IDI2 antibody [EPR17213]

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(3 Publications)

Rabbit Recombinant Monoclonal IDI1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Isopentenyl-diphosphate Delta-isomerase 1, Isopentenyl pyrophosphate isomerase 1, IPP isomerase 1, IPPI1, IDI1

4 Images
Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)
  • WB

Unknown

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Blocking and diluting buffer was 5% NFDM /TBST.

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)
  • WB

Supplier Data

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Blocking and diluting buffer was 5% NFDM /TBST

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal skeletal muscle lysate at 10 µg

Lane 3:

Human colon cancer lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)
  • WB

Unknown

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Blocking and diluting buffer was 5% NFDM /TBST.

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with IDI1 peptide

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with IDI2 peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)
  • WB

Supplier Data

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617)

Blocking and diluting buffer was 5% NFDM /TBST

Exposure time Lane 1 and 2 : 2minutes;
Lane 3 : 3minutes

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 2min

  • Carrier free

    Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17213

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The isopentenyl-diphosphate delta isomerase 1 also known as IDI1 and its paralog IDI2 are enzymes that play an important role in isoprenoid biosynthesis. IDI1 has a molecular weight of approximately 27 kDa. Both IDI1 and IDI2 facilitate the interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) two critical intermediates in the mevalonate pathway. These enzymes express widely across different human tissues with notable expression in the liver brain and skeletal muscle.
Biological function summary

IDI1 and IDI2 contribute to the regulation of cholesterol and other sterol biosynthesis by converting linear five-carbon units into branched forms necessary for further pathway reactions. Although not usually part of a larger complex these enzymes directly impact the supply of precursors for the synthesis of essential biomolecules such as steroids dolichols and ubiquinones. This transformation is not only pivotal for maintaining lipid homeostasis but also for facilitating cellular proliferation and differentiation.

Pathways

IDI1 and IDI2 should be considered during discussions on the mevalonate pathway which is an important component of cholesterol synthesis and other essential biomolecule formation pathways. These enzymes interact with other proteins involved in the mevalonate pathway including HMG-CoA reductase which functions upstream of IPP and DMAPP isomerization. IDI1 and IDI2 function vitally to ensure a balance between IPP and DMAPP affecting the synthesis rates of downstream isoprenoids and sterols essential for cell membrane structure and signaling.

Dysregulation of IDI1 and IDI2 has connections to metabolic disorders such as dyslipidemia and certain types of cancer. Altered expression or mutation in these enzymes could disrupt normal cholesterol synthesis potentially contributing to high lipid levels in the blood as seen in dyslipidemia. Furthermore IDI1 connects to oncogenesis possibly contributing to cancer development by aiding in producing isoprenoid derivatives critical for tumor cell proliferation. In these situations the enzymes interact closely with proteins regulating cell growth like Ras through their role in providing necessary lipid modifiers for membrane anchoring and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).
See full target information IDI1

Additional targets

Isopentenyl-diphosphate delta-isomerase 2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular biomedicine 6:78 PubMed41071519

2025

Lipin3 deficiency promotes hepatocyte ferroptosis and pyroptosis via activating JAK1-STAT3 pathway during acetaminophen induced acute liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Xing Liu,Qian Wang,Zi-Yu Xiangyang,Jie-Yi Long,Hao Huang,Liang-Liang Fan

Cell death & disease 15:704 PubMed39349433

2024

Cholesterol inhibition enhances antitumor response of gilteritinib in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Yue Sun,Di Cao,Yue-Ning Wang,Nuo-Qing Weng,Qian-Nan Ren,Shuo-Cheng Wang,Mei-Yin Zhang,Shi-Juan Mai,Hui-Yun Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e2204711 PubMed36307901

2022

Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Yun Peng,Dian-Kui Cai,Ren-Li Zeng,Chao-Yang Zhang,Guan-Cheng Li,Si-Fan Chen,Xiao-Qing Yuan,Li Peng
View all publications
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