Skip to main content

Rabbit Recombinant Monoclonal IDI1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617), expandable thumbnail
  • Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617), expandable thumbnail
  • Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617), expandable thumbnail
  • Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (AB205617), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).

Additional Targets

Isopentenyl-diphosphate delta-isomerase 2

Alternative names

Isopentenyl-diphosphate Delta-isomerase 1, Isopentenyl pyrophosphate isomerase 1, IPP isomerase 1, IPPI1, IDI1

Recommended products

Rabbit Recombinant Monoclonal IDI1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17213
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The isopentenyl-diphosphate delta isomerase 1 also known as IDI1 and its paralog IDI2 are enzymes that play an important role in isoprenoid biosynthesis. IDI1 has a molecular weight of approximately 27 kDa. Both IDI1 and IDI2 facilitate the interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) two critical intermediates in the mevalonate pathway. These enzymes express widely across different human tissues with notable expression in the liver brain and skeletal muscle.

Biological function summary

IDI1 and IDI2 contribute to the regulation of cholesterol and other sterol biosynthesis by converting linear five-carbon units into branched forms necessary for further pathway reactions. Although not usually part of a larger complex these enzymes directly impact the supply of precursors for the synthesis of essential biomolecules such as steroids dolichols and ubiquinones. This transformation is not only pivotal for maintaining lipid homeostasis but also for facilitating cellular proliferation and differentiation.

Pathways

IDI1 and IDI2 should be considered during discussions on the mevalonate pathway which is an important component of cholesterol synthesis and other essential biomolecule formation pathways. These enzymes interact with other proteins involved in the mevalonate pathway including HMG-CoA reductase which functions upstream of IPP and DMAPP isomerization. IDI1 and IDI2 function vitally to ensure a balance between IPP and DMAPP affecting the synthesis rates of downstream isoprenoids and sterols essential for cell membrane structure and signaling.

Associated diseases and disorders

Dysregulation of IDI1 and IDI2 has connections to metabolic disorders such as dyslipidemia and certain types of cancer. Altered expression or mutation in these enzymes could disrupt normal cholesterol synthesis potentially contributing to high lipid levels in the blood as seen in dyslipidemia. Furthermore IDI1 connects to oncogenesis possibly contributing to cancer development by aiding in producing isoprenoid derivatives critical for tumor cell proliferation. In these situations the enzymes interact closely with proteins regulating cell growth like Ras through their role in providing necessary lipid modifiers for membrane anchoring and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617), expandable thumbnail

    Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617)

    Blocking and diluting buffer was 5% NFDM /TBST.

    All lanes: Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with IDI1 peptide

    Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with IDI2 peptide

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

    Exposure time: 3min

  • Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617), expandable thumbnail

    Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617)

    Blocking and diluting buffer was 5% NFDM /TBST.

    All lanes: Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

    All lanes: Human fetal liver tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

    Exposure time: 3min

  • Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617), expandable thumbnail

    Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617)

    Blocking and diluting buffer was 5% NFDM /TBST

    All lanes: Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/1000 dilution

    Lane 1: Human fetal kidney lysate at 10 µg

    Lane 2: Human fetal skeletal muscle lysate at 10 µg

    Lane 3: Human colon cancer lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

    Exposure time: 3min

  • Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617), expandable thumbnail

    Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617)

    Blocking and diluting buffer was 5% NFDM /TBST

    Exposure time Lane 1 and 2: 2minutes;
    Lane 3: 3minutes

    All lanes: Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 3: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa

    Observed band size: 26 kDa

    Exposure time: 2min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com