AB251438

Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal IDI1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Isopentenyl-diphosphate Delta-isomerase 1, Isopentenyl pyrophosphate isomerase 1, IPP isomerase 1, IPPI1, IDI1, Isopentenyl-diphosphate delta-isomerase 2, Isopentenyl pyrophosphate isomerase 2, IPP isomerase 2, IPPI2, IDI2

4 Images

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Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (AB251438)

This data was developed using ab205617, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (<a href='/en-us/products/primary-antibodies/idi1-idi2-antibody-epr17213-ab205617'>ab205617</a>) at 1/5000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (AB251438)

This data was developed using ab205617, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (<a href='/en-us/products/primary-antibodies/idi1-idi2-antibody-epr17213-ab205617'>ab205617</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human fetal skeletal muscle lysate at 10 µg

Lane 3:

Human colon cancer lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Unknown

Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (AB251438)

This data was developed using ab205617, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (<a href='/en-us/products/primary-antibodies/idi1-idi2-antibody-epr17213-ab205617'>ab205617</a>) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with IDI1 peptide

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg with IDI2 peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (AB251438)

This data was developed using ab205617, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 2minutes; Lane 3 : 3minutes.

All lanes:

Western blot - Anti-IDI1 + IDI2 antibody [EPR17213] (<a href='/en-us/products/primary-antibodies/idi1-idi2-antibody-epr17213-ab205617'>ab205617</a>) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

true

Exposure time: 2min

Unconjugated

Anti-IDI1 + IDI2 antibody [EPR17213]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17213

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab251438 is the carrier-free version of ab205617.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDI1 (isopentenyl-diphosphate delta isomerase 1) and IDI2 (isopentenyl-diphosphate delta isomerase 2) are enzymes that catalyze the isomerization of isopentenyl diphosphate (IPP) to dimethylallyl diphosphate (DMAPP) an important step in the isoprenoid biosynthesis pathway. IDI1 is sometimes referred to as IPP isomerase. The molecular weight of IDI1 is approximately 33 kDa whereas IDI2 has a similar size. IDI1 is expressed in a wide range of tissues including the liver and heart. In contrast IDI2 has a more limited expression primarily found in skeletal muscle and testis.

Biological function summary

IDI1 and IDI2 are involved in the production of isoprenoids a diverse group of compounds that include cholesterol coenzyme Q and various hormones. These enzymes do not operate as part of a larger complex but instead function independently to facilitate the interconversion between IPP and DMAPP. The production of these molecules is essential for many physiological processes including cell membrane maintenance and hormone regulation.

Pathways

IDI1 and IDI2 play an integral role in the mevalonate pathway which is important for cholesterol biosynthesis and protein prenylation. In this pathway these enzymes help in providing substrates for key steps in the creation of cholesterol and other sterol molecules. IDI1 works closely with other enzymes like HMG-CoA reductase and squalene synthase which are key regulators within this same pathway.

IDI1 and IDI2 have connections to hypercholesterolemia and cardiovascular disease due to their roles in cholesterol biosynthesis. Altered activity or expression of these enzymes may lead to imbalances in cholesterol levels contributing to plaque formation in blood vessels. Therapeutic targeting of related enzymes like HMG-CoA reductase is already a strategy in managing these conditions highlighting the relevance of the isoprenoid pathway in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).

See full target information IDI1

Additional targets

Isopentenyl-diphosphate delta-isomerase 2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:26521 PubMed39489850

2024

GABPA inhibits tumorigenesis in clear cell renal cell carcinoma by regulating ferroptosis through ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqian Wu,Zonglong Wu,Mengfei Yao,Li Liu,Yimeng Song,Lulin Ma,Cheng Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free

Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (AB251438)

Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (AB251438)

Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (AB251438)

Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (AB251438)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise