Anti-IDO-2 antibody

• WB

Supplier Data

Western blot - Anti-IDO-2 antibody (AB131086)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO2 antigen affinity purified polyclonal antibody (Catalog # ab131086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

All lanes:

Western blot - Anti-IDO-2 antibody (ab131086) at 0.5 µg/mL

Lane 1:

A549 whole cell lysates at 50 µg

Lane 2:

Human placenta tissue lysates at 50 µg

Lane 3:

Caco-2 whole cell lysates at 50 µg

Lane 4:

PC-3 whole cell lysates at 50 µg

Observed band size: 47 kDa

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